摘要
目的探讨中国华东地区汉族人群HLA—DRB1基因与斑秃发病、临床特点的关系。方法采用序列特异性引物PCR（PCR—SSP）技术对已确诊为斑秃的158例和正常人对照组172例进行HLA—DRB1基因多态性分析。并比较斑秃患者不同发病年龄、发病次数、病程、家族史及严重程度与HLA—DRB1基因的关联性。结果斑秃组HLA—DRB1＊03、HLA—DRB1＊11等位基因频率与对照组差异无统计学意义。斑秃组HLA—DRB1＊04（OR=1．99，PC=0．01）等位基因频率明显高于对照组。与正常人对照组比较，斑秃晚发组（发病年龄〉16岁）（OR=1．94，PC=0．02）、斑秃复发组（发病次数〉1）和初发组（OR=2．49、PC=0．02，OR=1．83、PC=0．04）、病程〉1年的患者（OR=2．94，PC=0．01）、无家族史的患者（OR=1．97，PC=0．02）、严重斑秃患者（OR=3．53，PC=0．00）HLA—DRB1＊04等位基因频率均显著升高。结论中国华东地区汉族人群HLA—DRB1＊04等位基因与斑秃发病、临床分型显著相关。
Objective To investigate the association of HLA-DRB1＊03, ＊04 and ＊11 alleles with alopecia areata （AA） in Han Nationality in East China. Methods Polymerase chain reaction-sequence specific primer （PCR-SSP） method was conducted in 158 Chinese Han patients with AA as well as in 172 healthy human controls in East China. The relationships of HLA-DRB1 polymorphism to age of onset, episode frequency, clinical course, family history, and severity of AA were evaluated. Results No significant differences were observed for the frequency of HLA DRB 1＊03, ＊ 11 alleles between the patients and human controls, while increased frequency of HLA-DRBI＊04 was observed in patients （OR = 1.99, Pc = 0.01 ）. Multiple logistic regression analysis revealed that HLA-DRB1＊04 was more prevalent in patients with an onset after 16 years of age （OR = 1.94, Pc = 0.02）, those without family history （OR = 1.97, Pc = 0.02）, those with recurrent AA （OR = 2.49, Pc = 0.02）, those with a clinical course of more than 1 year （OR = 2.94, Pc = 0.01 ）, those with severe AA （OR = 3.53, Pc = 0.00） and those with single episode of AA （OR = 1.83, Pc = 0.04） in comparison with the normal human controls. Conclusion This study demonstrates that HLA-DRB1 ＊04 allele is associated with the occurrence and clinical types of AA in Han Nationality in East China.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2008年第12期793-795,共3页
Chinese Journal of Dermatology
