摘要
目的 探讨胆固醇侧链裂解酶CYP11α基因微卫星多态性与多囊卵巢综合征(polycysticovary syndrome,PCOS)患者高雄激素血症形成的关系。方法 应用聚合酶链反应及聚丙烯酰胺凝胶电泳分离技术检测86例PCOS患者及5 0名对照妇女CYP11α基因微卫星多态性及其分布,比较CYP11α(tttta) n各等位基因与PCOS高雄激素之间的关系。结果 CYP11α基因有4种等位基因(4、6、8、9) ;PCOS及对照组基因频率分布为0 .17,0 .31,0 .39,0 .13和0 .2 2 ,0 .35 ,0 .33,0 .10 ,差异无统计学意义;CYP11α(tttta) n各等位基因与PCOS高雄激素无明显关系。结论 中国汉族CYP11α微卫星多态性对PCOS高雄激素血症的形成无明显关系,不是PCOS的主要致病因素。
Objective To explore the association between the microsatellite polymorphism in the promoter region of CYP11α gene and hyperandrogenism of polycystic ovary syndrome (PCOS). Methods Eighty-six cases of PCOS and 50 normal women as controls were studied. Polymerase chain reaction and electrophoresis on polyacrylamide gel were employed to detect the polymorphism of CYP11α gene and its frequency distribution. At the same time, the relationships of CYP11α alleles to serum testosterone levels in PCOS were compared. Results Four different CYP11α (tttta)n alleles were identified, corresponding to 4,6,8 and 9 repeat-units alleles. The frequency distribution profiles were 0.17, 0.31, 0.39, 0.13 and 0.22, 0.35, 0.33, 0.10 in PCOS group and control group respectively, showing no statistically significant difference between the two groups. There were no correlations between the polymorphism of CYP11α gene and the serum testosterone levels of PCOS patients. Conclusion Microsatellite polymorphism (tttta)n of gene CYP11α exists in Chinese women and the polymorphism does not relate to the pathogenesis of hyperandrogenism in women with PCOS.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2005年第2期216-218,共3页
Chinese Journal of Medical Genetics
基金
河南省科技厅科技攻关资助项目(0424410097)~~
