摘要
采用超声波法提取茶皂素的工艺,确定最佳的工艺参数为:乙醇浓度80%、料液比1:4、提取温度40℃、超声波功率320W、超声波作用时间60min。最佳工艺条件下的茶皂素粗品得率为18.54%,茶皂素含量60.3%。用硅胶层析法对粗品进行分离纯化。能将茶皂素粗品中的杂质有效地分离,适用于茶皂素的分离纯化。
In this study, the optimal ultra-sonic extraction conditions of tea saponin （TS） are determined as below： ethanol concentration 80%, ratio of material-liquid 1：4, extraction temperature 40℃, ultra-sonic powder 320 W, and extraction time 60 min. Under these conditions, the yield of crude TS is 18.54%, and the content of TS is 60.3%. Then the crude TS was further purificated and separated by silica gel column. The results showed that silica gel chromatograph can efficiently separated TS from impurities, which is suitable to purificate TS.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第11期154-156,共3页
Food Science
基金
上海海洋大学研究生科研基金项目(科07-271-136)
关键词
茶皂素
提取
超声波
硅胶柱层析色谱
纯化
tea saponin
extraction
ultra-sonic
silica gel chromatograph
purification
