摘要
以油茶粕为原料,以茶皂素含量为指标,利用黑曲霉固态发酵降解茶皂素。通过单因素实验确定初始含水量70%,发酵时间84h,添加0.01mol/LHCl溶液4mL,发酵温度33℃,接种量10~7个单孢子/mL,在此条件下茶皂素含量由20.38%降低为3.06%。通过响应面实验对初始含水量、发酵温度、pH、发酵时间4个因素进行优化,其最佳工艺条件为发酵温度34.5℃,初始含水量70%,发酵时间84h,pH4.35,其它条件不变,茶皂素含量为2.04%,降解率达到89.99%。
With oil-tea-dregs as raw material,with tea saponin content as the norm,we used Aspergillus niger degradating tea saponin by solid-state fermentation.Through the single factor experiment,we determined the initial moisture content 70%,fermentation time 84 h,initial add 0.01 mol/L 4 mL,HCl solution,fermentation temperature 33 ℃,inoculation quantity 10~7 single spore/mL.tea saponin content decreased to 3.06% by 20.38% in these conditions;Further by the response surface experiment we studied the initial water content,fermentation temperature,pH,fermentation time.Through the analysis tea saponin content was 2.04%,degradation rate was 89.99% with the final optimum technological conditions is fermentation temperature 34.5 ℃,the initial moisture 70 percent,fermentation time 84 h,and pH 4.35,other conditions remained unchanged.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2012年第6期71-79,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家"十一五"科技支撑项目(2009BADB1B10)
湖南省生物学重点学科建设项目资助
关键词
茶皂素
黑曲霉
固态发酵
降解
Tea saponin
Aspergillus niger
solid-state fermentation
degradation
