摘要
目的：研究癌基因HER-2、端粒酶（the human telomerase gene，hTERC）在不同程度子宫颈病变脱落细胞中的表达，以探讨其在子宫颈癌发生、发展中的作用及临床意义。方法：采用荧光原位杂交（fluorescent in situ—hybridization，FTSH）方法，检测15例正常、76例子宫颈上皮内瘤变（CIN）及24例浸润性子宫颈癌脱落细胞中HER-2、hTERC的表达情况。结果：①HER-2在正常子宫颈脱落细胞、CINⅠ、CINⅡ-Ⅲ及浸润性子宫颈癌中的阳性表达率分别为0．0％、6．3％、16．6％及58．3％，hTERC基因阳性表达率为6．7％、18．8％、65．0％及100．0％。②HER-2、hTERC基因在CINⅠ、CINⅡ-Ⅲ中阳性表达率比较，差异无显著性（P〉0．05）；在CINⅡ-Ⅲ及子宫颈癌组阳性表达率均显著高于正常组（P〈0．05），同时子宫颈癌组明显高于CIN组（P〈0．05）。③HER-2高表达与细胞分级有明显相关性（P〈0．05），与临床分期、病理类型无关。结论：HER-2、hTERC基因高表达在子宫颈癌发生、发展中可能起重要作用，在子宫颈病变脱落细胞中检测HER-2、hTERC基因可以作为子宫颈癌早期筛查、病情监测和评估预后的辅助指标。
Objective： To investigate the expression of the HER -2 and hTERC oncogene in cervical scraping samples, and to evaluate the effect and clinical significance of HER -2 and hTERC in cervical lesion. Methods： The fluorescent in situ hybridization （FISH） was used to detect the expression of HER -2 and hTERC oncogene in cervical scraping samples in 24 cases with infiltrating cervical cancer, 76 cases with cervical intraepithelial neoplasia and 15 cases with normal control cervix. Results： ①Positive expression rates of HER -2 in normal cervixes, CINⅠ、CINⅡ-CINⅢ and infiltrating cervical cancer were 0. 0%, 6. 3%, 16. 6%, 58. 3%, respectively. The equivalent positive expression rates of the hTERC gene were 6. 7%, 18. 8%, 65. 0% and 100. 0%. ②There was no significant difference between CINⅠand CINⅡ-CINⅢ （P 〉 0. 05）. Compared with control cervix, positive expression of the HER - 2 and hTERC in CINⅡ-Ⅲ and infiltrating cervical cancer were different significantly （P 〈 0. 05 ）, and the expression in CIN was lower than that in infiltrating cervical cancer （P 〈 0. 05）. ③The over expression of HER - 2 was correlated with the differentiation degree of carcinoma closely （ P 〈 0. 05）. No correlation was found between positive expression and clinical stages, nor pathologic types. Conclusion： The over expression of HER - 2 and hTERC gene may play an important role in cervical carcinogenesis, the HER - 2 and hTERC gene of cervical scraping samples could be used as a better tumor index for early cervical cancer screening and predicate the the advancement and evaluate poor prognosis of cervical leision.
出处
《中国妇幼保健》
CAS
北大核心
2008年第33期4744-4748,共5页
Maternal and Child Health Care of China
基金
卫生部医药卫生科技发展中心项目(项目编号:WKJ2007-3-001)
关键词
HER-2基因
端粒酶
宫颈癌
荧光原位杂交
子宫颈瘤变
HER -2 gene
hTERC gene
Cervical cancer
Fluorescence in situ hybridization （FISH）
Cervical intraepithelial neoplasia
