摘要
目的:探讨NGX6mRNA表达与肝细胞癌超声造影特征的关系。方法:45例病理证实的肝细胞癌患者术前行超声造影,应用RT-PCR检测NGX6在癌组织中的表达,分析NGX6表达和超声造影灌注时间变化以及声像特征的关系。结果:肝细胞癌NGX6mRNA表达阳性组与阴性组之间造影时间变化差异不明显;NGX6mRNA表达与肿瘤强化边缘(χ2=10.499,P<0.01,列联系数C=0.435),强化范围面积变化(χ2=5.867,P<0.05,列联系数C=0.34),癌栓强化发生(χ2=6.749,P<0.01,列联系数C=0.361)有关。与廓清模式、放射状滋养动脉、强化是否完全等特征无关。结论:NGX6低表达与肝细胞癌浸润、转移关系密切,超声造影可提供影像学依据,对于指导基因靶向治疗有重要作用。
Objective： To study the correlation of contrast-enhanced ultrasound features with the expression of nasopharyngeal carcinoma associated gene 6 （NGX6） in patients with hepatocellular carcinoma. Methods： A total of 45 patients with hepatocellular carcinoma confirmed by surgery and pathology were recruited. The entire contrast-enhanced ultrasound procedures were recorded and analyzed by QONTRAST-QRADIO software. Fresh tissue samples were collected during surgery. Reverse transcription polymerase chain reaction was used to detect the expression of NGX6 in samples of hepatocellular carcinoma. These 45 cases were divided into two groups： the group with positive NGX6 expression （group 1） and the group without NGX6 expression （group 2）. Results： Positive NGX6 expression was found in 16 （35.5%） of the 45 samples of hepatocellular carcinoma. Contrast-enhanced ultrasound showed a quick wash-out pattern in 32 cases and a slow wash-out pattern in 13 cases. An enlarged enhancement range was observed in 30 cases. Twenty-eight cases had clear margins and 17 cases had dim margins. Twenty-six cases had a complete enhancement and 19 cases had an incomplete enhancement. Thirty-eight cases had radical nourishing artery flow and 17 cases had portal vein tumor thrombus enhancement. The mean initiation time of enhancement, peak time, peak intensity, and the time of hepatocellular carcinoma becoming iso-enhancing and hypo-enhancing were 14.2±3.9s, 13.1±3.5s, 26.6±4.12s, 24.5±5.27s, and 51.3± 15.4% in Group 1 and 54.2±14.3%, 36.8±7.2s, 33.9±9.4s, 48.3±9.4s, and 46.5±7.1s in Group 2, respectively. No significant difference was found in perfusion time and peak intensity between the two groups. Pearson correlation analysis found no significant correlation between the variation time of contrast-enhanced ultrasound or its peak intensity and the expression of NGX6 mRNA （P〉0.05）. The expression of NGX6 mRNA was correlated with tumor margin （x^2=10.499, P〈0.01, contingency coefficient=0.435）, range of
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2008年第21期1228-1232,共5页
Chinese Journal of Clinical Oncology
基金
湖南省卫生科技计划基金资助(编号:B2005073)~~
关键词
肝细胞癌
鼻咽癌相关基因6
超声造影
Hepatocellular carcinoma
Nasopharyngeal carcinoma associated gene 6 （NGX6） Contrast-enhanced ultrasound
