摘要
目的:建立结肠癌肝转移模型,为抑瘤基因NGX6功能研究提供体内试验平台。方法:将低分化结肠癌细胞系HT-29组(HT-29)、空白质粒转染组[pcDNA3.1(+)/HT-29]、NGX6转染组[(pcDNA3.1(+)/NGX6/HT-29)]细胞分别接种于裸鼠脾脏下极包膜内,每天称量裸鼠体质量并观察裸鼠摄食、活动及精神情况,45d后处死裸鼠,分别从大体水平以及镜下观察肝、脾、肺、肾、脑等器官肿瘤转移情况。结果:pcDNA3.1(+)/NGX6/HT-29组裸鼠较其他两组裸鼠肝脏转移灶数目明显减少、脾脏上种植瘤形成明显减少。结论:抑瘤基因NGX6抑制结肠癌HT-29细胞的转移,裸鼠脾内种植转移模型可作为新基因体内功能研究的理想模型。
Objective To establish a liver metastasis model of nude mice in colon cancer so as to determine the function of NGX6. Methods The cells of Group HT-29, pcDNA3. 1 （＋）/ HT-29, and pcDNA3. 1 （＋）/NGX6/HT-29 were implanted into the spleen of nude mice, respec-tively. Everyday we measured the weight of the nude mice and observed their ingestion, movement and mental status. The nude mice were killed after 45 days, and the effect of NGX6 on the malig-nant behavior of HT-29 was assessed by this experiment. Results In contrast to the other two groups, the metastasis in the liver and xenograft tumor in the spleen of pcDNA3. 1 （ ＋ ）/NGX6/ HT-29 group was significantly reduced （P 〈 0.01 ）. Conclusion The metastasis of HT-29 colon cancer cell line was significantly inhibited by NGX6 gene. This model of liver metastasis in the nude mice is a proper model to determine the anti-metastasis mechanism of NGX6 gene.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2007年第5期753-757,共5页
Journal of Central South University ：Medical Science
基金
国家自然科学基金(3037081
30500238)
湖南省自然科学基金(04JJ3109
06JJ20068)
国家教育部博士专项科研基金(20040533062)~~
