摘要
以马尾松为试验材料,采用邻苯二甲醛-9-芴基甲基氯甲酸酯(OPA-FMOC)柱前衍生,高效液相色谱法定性定量地检测马尾松毛虫取食、外源茉莉酸甲酯(MeJA)和萜烯混合物处理4h后损伤针叶、姊妹针叶(处理对枝)、系统上枝(处理上枝)针叶和系统下枝(处理下枝)针叶内γ-氨基丁酸(GABA)和17种游离氨基酸含量的变化情况。结果显示,不同受害方式对马尾松苗木体内GABA和17种游离氨基酸的系统性变化有不同的影响。虫害、10μmol·L-1茉莉酸甲酯和10μmol·L-1萜烯混合物熏蒸,损伤枝、姊妹枝和系统枝中GABA含量明显升高;虫害和10μmol·L-1茉莉酸甲酯熏蒸使马尾松损伤枝、姊妹枝和系统枝中的氨基酸总量降低;10μmol·L-1萜烯混合物熏蒸,马尾松熏蒸枝和系统下枝氨基酸总量略有下降,姊妹枝和系统上枝氨基酸总量升高。证明茉莉酸甲酯和萜烯类物质作为植物体内伤信号的报警信号分子,能代替虫害等外界刺激启动相似的信号防御系统,起到防御外界伤害的作用。
Pinus massoniana seedling was used to investigate effects of Dendrolimus punctatus chewing, MeJA and terpenes treatments on the contents of γ-aminobutyric acid and 17 free amino acids with the technology of O-phthaldialdehyde-9- fluorenylme-thyl chloroformate(OPA-FMOC) precolumn derivation, along with high performance liquid chromatography gradient elution method. The damaged shoots, the sister shoots (the opposite), the systematic upper shoots (above the damaged shoots) and the systematic lower shoots (under the damaged shoots) were sampled after being injured by the insects, being treated by the MeJA and the terpenes for 4 hours. Results showed that different modes of damage considerably and systematically caused the changes of GABA and 17 free amino acids in P. massioniana seedlings. Contents of GABA increased distinctly in the shoots damaged by the insect, treated with MeJA ( 10 μmol· L^- 1 ) and terpenes ( 10 μmol· L^- 1 ), and in their sister shoots and the systematic shoots. The total contents of amino acids decreased in the leaves of the shoots chewed by the insect and treated with MeJA ( 10 μmol· L^- 1 ), and of the sister shoots and the systematic shoots. The total contents of amino acids decreased in the shoots treated with terpenes (10 μmol· L^-1 ) and the systematic lower shoots, however, increased in the sister shoots and the systematic upper shoots. It was concluded that MeJA and terpenes induced the similar defensive systems as the insect did, and could replace the insect to signal and initial the defensive system to defend against the outer attack.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2008年第10期69-75,共7页
Scientia Silvae Sinicae
基金
国家自然科学基金重点资助项目(30330490)
国家林业局重点项目(2006-59)
国家"十一五"科技支撑计划课题(2006BAD01A15
2006BAD24B04)