摘要
目的了解尼麦角林在慢性脑缺血治疗中脑源性神经营养因子(BDNF)和神经生长因子(NGF)阳性表达的意义,为早期慢性脑缺血防治寻找对策。方法采用颈动脉线结扎离断法制作大鼠的局灶性慢性脑缺血模型,脑缺血2周给尼麦角林1mg/kg、尼莫地平1mg/kg,腹腔注射1次/d。分别于缺血后4、8、12、16周观察脑组织中BDNF和NGF的阳性表达细胞数。结果尼麦角林和尼莫地平治疗后在大脑额叶皮质、海马CA1区BDNF和NGF阳性表达细胞数显著高于对照组和缺血组,P<0.01。对照组和缺血16周时缺血组、尼莫地平组、尼麦角林组BDNF阳性表达细胞数额叶皮质分别为9.31±0.52、8.73±0.96、14.62±1.52、17.35±1.31,海马CA1区分别为8.26±0.47、7.56±1.47、13.46±1.92、15.52±1.59;NGF阳性表达细胞数额叶皮质分别为10.54±1.65、8.73±0.96、13.08±1.52、15.54±1.31,海马CA1区分别为8.35±0.87、8.22±1.47、13.46±1.92、16.38±1.59,,且尼麦角林组又较尼莫地平组明显,P<0.05。结论慢性脑缺血后大脑额叶皮质、海马CA1区BDNF和NGF阳性表达细胞数增多,有利于减少细胞凋亡,保护受损神经元。尼麦角林和尼莫地平均有上调BDNF和NGF蛋白表达作用。
Objective To investigate the significance of nicergolent on expression of BDNF and NGF in chronic cerebral is chemia, and search a strategy for treatment to earlier period chronic cerebral ischemia. Methods Chronic global ischemia model was established by occluding bilateral carotid of rats,nicergolent （1mg/kg）,and nimodipine （1mg/kg）were peritoneal injected,qd. two weeks after cerebral ischemia,The injury of rats nerve function were evaluated by apoplexy index score standard and neurology score standard. The amount of the neurons with expression of BDNF and NGF in brain tissue was observed by immunity pathological section-staining after ischemia for 4,8,12,16 hours respectively. Results There was a manifestation with the apoplexy index and nerve symptoms score that rat＇s death rate were 35%,38.7% at ld,3d after ischemia,which were lower than those in the control group, （P〈0.05）. The amount of the .neurons with expression of BI）NF and NGF in cortex of frontal lobe and hippocamp CA1 region were higher than those in the control group after the treatment of nicergolent and nimodipine. The amount of the neurons in the nicergolent-treated group were significantly higher than those in the nimodipine-treated group, （P〈0.05）. Conclusions The neurons with expression of BDNF and NGF in cortex of frontal lobe and hippocamp CA1 region after chronic cerebral ischemia were markedly increase,which decreased apoptosis and protected neurons from damaged. Nicergolent and nimodipine could up-regulate the expression of BDNF and NGF.
出处
《中华保健医学杂志》
2008年第4期265-267,共3页
Chinese Journal of Health Care and Medicine
