摘要
为制备具有抗菌斑和抗胶原酶特性的引导牙周组织再生（ＧＴＲ）胶原膜，作者将普通交联胶原膜用四环素（ＴＣ）缓释装置包被，制成双抗胶原膜（ＢＡＣＭ）。检测结果：ＢＡＣＭ具均匀小网孔；低应变区弹性模量和膨胀率分别为２０４ｇ／ｍｍ２和０１４１；豚鼠过敏试验阴性；抗１００ｕ胶原酶消化时达３０ｄ以上；１ｍｇＢＡＣＭ植入小鼠皮下可维持７ｗ左右；ＢＡＣＭ（含ＴＣ１５０μｇ）置入牙周袋内７ｄ，龈沟液ＴＣ浓度维持在４６７６μｍｏｌ／Ｌ±５．６９μｍｏｌ／Ｌ；植入翻瓣术患者根面７ｄ，其上附着菌明显少于对照膜。结果提示具有双抗效应的ＢＡＣＭ用于ＧＴＲ将发挥更好的作用。
This study was undertaken to assess the antiplaque and anticollagenase properties of bianticollagen membrane (BACM) for use in the guided tissue regeneration (GTR). First, Preparaing crosslinked collagen membrane (GLCM) from bone collagen by glutaraldehyde and ultraviolet irradiation. Then, the GLCM was coated with tetracycline (TC) delivery device, which is BACM. BACM's properties are as follows: under scan electron microscope (SEM), it is a three dimensional structure with small pores; the modulus of elasticity in low strain regions and swelling ratio are 20.4 g/mm2 and 0.141 respectively; immediate type and delayed type hypersensitivity are negative; BACM with TC 150 μg placed into pockets, the average intrasulcular TC concentration measured at the end of the 7days is 46.76±5.69 μmol/L, Which is 2 times higher than MIC of TC; the period against collagenase (100u) digestion is over 30 days in vitro; 1 mg GLCM and BACM implanted in mice may maintain about 4 weeks and 7 weeks respectively, and BACM and GLCM placed onto patient′s root surface in flap operation and taken at 7th day the bacteria on BACM are significantly less than that on GLCM under SEM. These results indicate that BACM has antiplaque and stronger antidegradation effects than GLCM.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1997年第6期369-371,共3页
Chinese Journal of Stomatology
关键词
引导组织再生
胶原
四环素
牙周炎
Guided tissue regeneration Collagen Tetracycline Periodontitis
