摘要
应用循环伏安法、微分脉冲伏安法和紫外光谱法研究了6-苄氨基嘌呤（6-BA）在汞电极上的电化学行为，及与小牛胸腺DNA的相互作用。结果发现，6-BA的循环伏安曲线显示三对扩散或吸附控制的氧化还原波。扩散波的氧化峰电流随6-BA浓度在0．1～50．0μmol·L^-1范围内呈现良好的线性关系。依据预吸附时间和溶液PH值对吸附波的还原峰电位和峰电流的影响，讨论了6-BA在汞电极上的咐附机理。另外，6-BA的还原峰电流随DNA浓度的增加而减小，峰电位正移，紫外吸收峰出现明显的减色效应，认为6-BA通过部分插入作用与DNA结合，结合常数为2．3×10^3L·mol^-1。
The electrochemical behavior of 6-benzylaminopurine（6-BA） on a mercury electrode has been investigated using cyclic voltammetry, differential pulse voltammetry and UV spectroscopy. It is found that there are three pairs of redox waves for 6-BA on the mercury electrode controlled by adsorption or diffusion process. Based on the effect of accumulation time and pH values on the cathodic peak potentials and currents of adsorption controlled waves, their adsorption mechanism is discussed. The anodic peak currents of diffusion-controlled waves increase with increasing concentration of 6-BA. A good linear relationship is exhibited in the concentration range from 0. 1 to 50. 0 μmol · L^-1. The cathodic peak currents of 6-BA decrease and peak potentials shift positively with increasing amounts of DNA. In addition, the absorption bands of 6-BA are regularly changed in the presence of DNA, the intensity is weakened and the maximal absorption peak value red-shifted. The binding constant of 6-BA with DNA is 2.3 × 10^3 L·mol^-1. Their interactive mode is suggested to be via the part intercalation.
出处
《分析科学学报》
CAS
CSCD
2008年第5期535-539,共5页
Journal of Analytical Science
基金
广东省教育厅自然科学基金(Z03020)
广州市科技攻关项目(2004J1-C0091)
