摘要
通过外施不同浓度的细胞分裂素类物质 6- BA( 6-苄氨基嘌呤 ) ,对 Hg2 + 污染下的水花生 ( Alternan-thera philoxeroides( Mart.) Griseb.)抗氧化酶系的变化进行了研究 ,结果发现 :不同浓度的 6- BA对重金属毒害均有缓解作用。过氧化物酶 ( POD)、超氧化物歧化酶 ( SOD)、过氧化氢酶 ( CAT)的活性均不同程度的提高 ,丙二醛 ( MDA)及活性氧 ( O· - 2 )含量的增加变慢。表现在外部形态上 ,6- BA延缓了叶片失绿、腐烂过程 ,新根生长受抑制的现象在低 Hg2 + 浓度下也明显缓解 ;不同浓度的 6- BA作用效果不同 :在 Hg2 + 毒害下 ,2× 1 0 - 5mol/L6- BA对 SOD、MDA和 O· - 2 伤害的缓解效果最好 ,而 POD、CAT的破坏在 3× 1 0 - 5mol/L浓度下缓解效果最显著 ;6- BA缓解重金属毒害的作用可能与调节活性氧代谢及蛋白质的表达有关。
More attention has been paid to Cytomin, one of the activest members of phytohormone, since found in 1960s. Recently, the studies on cytomin are mainly focused on the field of tissue culture and regulation of gene expression, etc. and a few have been done on anti-adversity (such as drought, waterlog and salt stress) and antiaging. But, because of atmospheric sedimentation, geological leakage, untreated industrial effluents, and waste dischargement, heavy metals have become one of the primary factors that bring about global pollution. Among them, heavy metal aquatic pollution can directly affect the growth of hydrophyte. Under this adversity, what effects will be produced by foliar spraying of cytomin on hydrophyte have not been reported in detail until now. In the present experiment, after cultivated with distilled water for two days, Alternanthera philoxeroides(Mart.)Griseb grows in aquarium containing different gradient concentration of Hg 2+. The experiment designs are as following: each five aquariums as one group, control group was only added with Hg 2+, and the other three ones were additional foliar sprayed with three different concentration of 6-BA(1×10 -5mol/L、2×10 -5mol/L、3×10 -5mol/L), respectively, to study the effect of spraying 6-BA on protective enzymes system(SOD、POD、CAT、MDA、O ·- 2,et al) compared with that of Hg 2+ treatment only. Through this experiment,we manage to find out the mechanism of Alternanthera philoxeroides in resisting heavy metal pollution and to provide methods and data against heavy metal pollution. The result shows that the activities of SOD、POD、CAT in Alternanthera philoxeroides rise firstly, then drop or continue to decrease with the increasing of Hg 2+ concentration, while the content of MDA and generation rate of O ·- 2 have a rising trend. After being sprayed with 6-BA, the obvious changes are found:the decrease of SOD、POD and CAT activities become much slower ,and MDA content and generation rate of O ·- 2 increase a little slower
出处
《生态学报》
CAS
CSCD
北大核心
2003年第2期387-392,共6页
Acta Ecologica Sinica
基金
国家自然科学基金资助项目 ( 39770 0 4 6)
江苏省教育厅自然科学基金资助项目~~
