摘要
目的:研究电针对慢性情绪应激(chronic emotional stress,CES)焦虑大鼠中枢单胺类神经递质与γ-氨基丁酸(γ-aminobutyric acid,GABA)失平衡的调节作用。方法:采用不可预知的情绪应激刺激方法,刺激大鼠21d,建立CES焦虑模型。电针"百会"、"三阴交"穴治疗21d,运用荧光分光光度法测定大鼠海马、大脑皮质单胺神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)和多巴胺(DA)的含量,用免疫组化法检测两脑区GABA的表达。结果:模型组较空白组大鼠海马和大脑皮质5-HT、NE和DA的含量明显升高、GABA平均光密度(AOD)值明显降低(P<0.05,或P<0.01);电针可降低模型大鼠两脑区单胺递质含量、上调其GABA表达(P<0.05或P<0.01)。结论:电针抗焦虑作用可能与调节两类神经递质间的失平衡相关。
Objective： To research the regulation effects of electro-acupuncture（EA）on the monoamine neurotransmitter and GABA in rat with chronic emotional stress-induced anxiety. Methods： Chronic emotional stress anxiety model was established by using chronic unpredictable emotional stress methods for 21 days. EA was applied to Baihui（GV20）and Sanyinjiao（SP6） and lasted for 21 days. The contents of 5-HT,NE and DA in the hippocampus and the cerebral cortex were measured by fluorospectrophotometry. The content of GABA in the hippocampus and the cerebral cortex were measured by immunohistochemistry. Results： Compared with control group,the contents of 5-HT,NE and DA increased obviously and the AOD value of GABA lowered significantly in model group（P〈0.05,or P〈0.01）. Compared with model group, the contents of 5-HT and NE and DA decreased obviously and the AOD value of GABA increased significantly in EA group（P〈0.05,or P〈0.01）. Conclusion： Antinxiety effect of EA is possibly related to modulation of the unbalanced monoamine neurotransmitter and GABA.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2008年第10期926-929,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
成都中医药大学针灸推拿学重点学科科研开放基金(No.20051007)
成都中医药大学校基金重点项目(No.ZRZD200502)
四川省教育厅自然科学重点项目(No.07ZA018)
