摘要
目的:研究微小染色体维持蛋白4(minichromosome maintenance proteins4,MCM4)、细胞分裂周期蛋白(cell divi-sion cycle6,CDC6)在宫颈癌组织中的表达及其与人乳头状瘤病毒16/18型(human papilloma virus16/18type,HPV16/18)感染的相关性及其临床意义。方法:收集2006-2007年间山西大同市第五人民医院病理科经病理证实的50例宫颈癌、20例宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)Ⅰ、20例CINⅡ-Ⅲ、20例正常宫颈组织石蜡标本,以免疫组织化学法检测这些组织标本中MCM4、CDC6的表达,同时采用PCR技术检测HPV16/18的感染情况。结果:(1)在宫颈癌组织中MCM4、CDC6表达的阳性率显著高于CIN和正常宫颈组织(均P<0.05),且MCM4、CDC6阳性表达率与宫颈癌病理分级和淋巴转移相关(均P<0.05),而与年龄分组、临床分期无关(均P>0.05)。(2)HPV16/18感染在正常宫颈组织、CIN和宫颈癌组织中的阳性率依次升高(P<0.05),但与宫颈癌患者年龄、临床分期、病理分级、淋巴转移无关(均P>0.05)。(3)宫颈癌组织中MCM4和CDC6的表达呈正相关(r=0.390;P<0.05);MCM4和CDC6表达均与HPV16/18感染相关(r=0.634,P<0.05;r=0.386,P<0.05)。结论:宫颈癌及CIN组织中MCM4、CDC6表达的改变与HPV16/18感染密切相关,共同影响CIN的发展及宫颈癌的发生与发展。
Objective ： To investigate the expression of miniehromosome maintenance proteins 4 （MCM4） and cell division cycle 6 （CDC6） in uterine cervical carcinomas and its relationship with human papilloma virus （HPV）16/18 infection. Methods：The expression of MCM4 and CDC6 was examined in 50 squamous cell carcinoma specimens, 20 cervical intra- epithelial neoplasia （CIN） Ⅱ -Ⅲ specimens, 20 CIN Ⅰ specimens, and 20 normal cervical tissues by immunohistochemieal method. The infections of HPV type 16, 18 DNA were determined by PCR. Results： （ 1 ） The expression of MCM4 and CDC6 in uterine cervical carcinoma tissues was significantly higher than that in the CIN specimens and normal cervical tissues （Both P 〈 0.05）. Expression of MCM4 and CDC6 was correlated with tumor grades and lymph node metastasis （all P 〈 0.05 ） , but not with age and clinical stages （all P 〉 0.05 ）. （2） The positive rates of （HPV）16/18 were significant different between cervical carcinomas, CIN and normal tissues （P 〈0.05） , and was not associated with age, tumor grades, clinical stages or lymphnode metastasis （ all P 〉 0.05 ）. （3） MCM4 expression were positively correlated with the expression of CDC6 in uterine cervical carcimonas（ r = 0. 390, P 〈 0.05 ）. The positive rate of HPV16/18 was positively correlated with the expression of MCM4 and CDC6 （r = 0. 634, P 〈 0.05 ; r = 0. 386 P 〈 0.05 ）. Conclusion ： The results suggest that changes in MCM4 and CDC6 expression in CIN and cervical carcinoma specimens are associated with HPV16/18 infection, which is related to the progression of CIN and carcinogenesis of cervical carcinomas.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2008年第4期374-378,共5页
Chinese Journal of Cancer Biotherapy
关键词
宫颈癌
微小染色体维持蛋白4
细胞分裂周期蛋白6
人乳头状瘤病毒
uterine cervical carcinomas
minichromosome maintenance proteins 4 （MCM4）
cell division cycle 6（CDC6）
human papillomavirus （HPV）
