摘要
目的:探讨宫颈癌及宫颈上皮内瘤变(CIN)中微小染色体维持蛋白7(MCM7)、细胞周期分裂蛋白6(CDC6)的表达及其意义。方法:应用免疫组织化学S-P法,检测宫颈癌31例、CIN50例、慢性宫颈炎10例中MCM7蛋白和CDC6蛋白的表达。结果:(1)MCM7在宫颈癌中的阳性表达率(93.6%)明显高于CIN(CINⅠ为60.0%,CINⅡ～Ⅲ为85.0%)和慢性宫颈炎(30.0%),差异有统计学意义(P<0.05);CDC6在宫颈癌中的阳性表达率(90.3%)明显高于CIN(CINⅠ为50.0%,CINⅡ～Ⅲ为77.5%)和慢性宫颈炎(30.0%),差异有统计学意义(P<0.05)。(2)在宫颈癌、CIN和慢性宫颈炎中,MCM7和CDC6的表达呈正相关(r=0.7356,P<0.05)。(3)MCM7的阳性表达率在宫颈癌不同分化程度间差异无统计学意义(P>0.05);CDC6在宫颈癌高分化组的阳性表达率(72.7%)明显低于中-低分化组(100.0%),差异有统计学意义(P<0.05);MCM7和CDC6的表达在不同年龄、民族间差异无统计学意义(P>0.05)。结论:MCM7和CDC6在宫颈癌的发生、发展中具有协同作用,对宫颈癌的早期诊断具有重要参考价值。
Objective： To investigate the expression and significance of MCM7 and CDC6 in cervical cancer and cervical intraepithelial neoplasia （CIN）. Methods： Immunohistochemical technique （S-P method）was used to detect the expression of MCM7 and CDC6 in 31 cases of cervical cancer, 50 cases of CIN and 10 cases of cervical chronic inflammation. Results： （1） The positive rate of MCM7 in cervical cancer （93.6%） was notably higher than that in CIN （CIN I was 60%, CIN Ⅱ～Ⅲ1 was 85.0%） and in cervical chronic in- flammation. The differences had significanees of statistics （P 〈0.05）. The positive rate of CDC6 in cervi- cal cancer （90.3%）was notably higher than in CIN （CIN I was 50.5%,CIN Ⅱ～Ⅲ was 77.5%） and cer- vical chronic inflammation （30.0%）. The differences had significance of statistics （P 〈0.05）. （2） There was a positive correlation between MCM7 and CDC6＇s expression in the cases of cervical cancer, CIN and cervical chronic inflammation （r = 0. 735 6,P〈0.05）. （3） The positive rate of MCM7 in different tumor grade did not have significance of statistics （P 〉0.05） ; The positive rate of CDC6 in G1 （72.7%） was lower than that in G2-3（100.0%）, it had significance of statistics （P〈0.05） ; the expression of MCM7 and CDC6 in different age and nation did not have significance of statistics （P 〈0.05）. Conclusions： MCM7 and CDC6 might have a cooperate value in the carcinogenesis and progression of cervical cancer, and they may play an important role in the diagnosis of cervical cancer in the early stage.
出处
《新疆医科大学学报》
CAS
2009年第12期1690-1692,共3页
Journal of Xinjiang Medical University
关键词
宫颈癌
宫颈上皮内瘤变
微小染色体维持蛋白7
细胞分裂周期蛋白6
cervical cancer
cervical intraepithelial neoplasia
minichromosome maintenance protein 7 （MCM7）
cell division cycle protein 6（CDC6）
