摘要
目的 分析不同方法及试剂检测乙型肝炎病毒血清标志物（HBV—M）的差异。方法我院门诊及住院患者48例，经雅培12000配套试剂检测为乙型肝炎表面抗原（HbsAg）阳性，乙型肝炎e抗原（HbeAg）测定值COI〈50。依据HBsAg定量．将患者分为：A组，HBsAg≥250IU/L，共16例；B组，HBsAg介于20～250IU／L，共10例；C组，HBsAg≤20IU／L．共12例。HBVM检测采用3种方法：（1）ARCHITECT12000型仝自动免疫分析仪及配套试剂；（2）ROCHEE1711型全自动免疫分析仪及配套试剂；（3）酶联免疫分析（ELISA）试剂。结果两种进口发光方法检测HBsAg全部吻合．但ELISA检测C组有4份为阴性。三种方法检测HBeAg与抗HBe差异明显．国产ELISA试剂对HBeAg与抗HBe检出率远远为低于发光法．对A组26份标本HBeAg全部漏检。三种方法检测48份血样抗-HBc的符合率为100％。结论 在HBV—M的5项指标中如有低值，使用不同试剂检测出现不同模式的机会升高。建议接受抗病毒药物治疗的患苕尽量选择存同一家医院并使用同一种试剂作长期观察。
Objective To analyse the difference between three methods of detecting HBV serum markers in pa tients with chronic HBV infection. Methods Totally 48 samples with three levels of HBsAg were selected for the detection. HBV serum markers were detected by ARCHITECT 12000 Chemi-luminescence lmmuno-Assay（CLIA）, ROCHE E170 Electro chemi luminescence Immuno-Assay （ECLIA）. and an Enzyme-Link Immunoabsorhent Assay（ELISA）. Results There were significant among the 5 HBV markers using the three methods, the ELISA showed the lowest sensitivity for HBeAg and HBeAb detection. Conclusion Voviiety of HBV serum markers is some time being a result of changing in methods or reagents. It is tee ommended that patients accepted anti virus treatment should detect HBV marker by the same method.
出处
《肝脏》
2008年第4期303-305,共3页
Chinese Hepatology
