摘要
采用显微拉曼光谱技术研究了亚硝酸钠(NaNO2)与氧合血红蛋白(HbO2)在水溶液中的相互作用,为检测高铁血红蛋白(Met Hb)提供了一种新的方法。激发波长514.5 nm,血红蛋白取自健康成人的血液。结果显示NaNO2与氧合血红蛋白反应后,氧合血红蛋白特征峰570 cm-1与水合高铁血红蛋白(Met HbH2O)特征峰495 cm-1强度的比值I570/I494,以及铁离子低自旋态与高自旋态特征峰强度的比值I1586/I1555均减小。对血红蛋白氧化态敏感的特征峰向低波数方向移动,对卟啉环中心孔径大小敏感的峰向高波数方向移动。研究表明拉曼光谱能够监测到NaNO2对血红蛋白的影响,鉴别血红蛋白与水合高铁血红蛋白,可作为一种检测高铁血红蛋白的新方法。
To explore a new method to dignose methemoglobin, micro-Raman spectroscopy was used to investigate the interaction of sodium nitrite and oxyhemoglobin in aqueous solution, with 514.5 nm exitation. Hemoglobin was obtained from the healthy adult. The results show that the inensity ratio of the oxyhemoglobin marker band 570 cm^-1 to the aquomethemoglobin marker band 495 cm^-1 , and that of low spin state marker band 1586 cm^-1 to the high spin state marker band 1555 cm^-1 decrease during the interaction. The bands sensitive to the oxidation state shift to higher frequency. This work indicates that micro Raman spectroscopy could monitor the bioeffects of sodium nitrite on the oxyhemoglobin, and distinguish hemoglobin and aquomethemoglobin. As a new method to detect methemoglobin, micro-Raman spectroscopy may have great potential applications in the diagnosis of methemoglobinemia.
出处
《中国激光》
EI
CAS
CSCD
北大核心
2008年第9期1432-1436,共5页
Chinese Journal of Lasers
