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血液样品中防腐剂对碳氧血红蛋白稳定性的影响 被引量:3

A study on the effect of preservatives on the stability of carboxyhemoglobin in the blood samples
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摘要 目的研究临床上常用8种试剂对血液样品中碳氧血红蛋白(HbCO)稳定性的影响。方法将血液样品分为高、低两个HbCO浓度组,选用临床常用的甲醛、氟化钠、乙二胺四乙酸二钠、亚硝酸钠、草酸钾、肝素钠、柠檬酸钾及氟化钠与草酸钾混合物(1:3)8种试剂,按常用浓度分别添加到血样品中,并于添加后0h、2h、8h、24h、3d、7d用紫外可见光分光光度法检测其中HbCO饱和度,用统计学方法进行结果分析。结果本实验选用的8种试剂只有甲醛和亚硝酸钠对HbCO的稳定性影响较为显著,而其余6种对HbCO稳定性的影响无统计学意义。结论在检验疑似CO中毒并经甲醛或亚硝酸钠防腐的检材时应慎重,以免导致错误的鉴定结论。 Objective To investigate the effect of conventional preseratives on the stability of carboxyhemoglobin(HbCO) in the stored blood samples.Methods Blood samples with 30%~40% and 60%~70%of HbCO were established,respectiviely.The samples were stored with the commomly used preseratives including formaldehyde,sodium fluoride,EDTA-Na2,sodium nitrite,potassium oxalate,heparin sodium,sodium citrate and the mixture of sodium fluoride and sodium citrate(1∶3).Saturation of HbCO in the preserved blood samples were deter...
出处 《中国法医学杂志》 CSCD 北大核心 2010年第1期20-23,共4页 Chinese Journal of Forensic Medicine
基金 "十一五"国家科技支撑计划项目(2007BAK26B05) 山西省高等学校中青年拔尖创新人才支持计划资助(TYMIT)
关键词 法医毒物分析 一氧化碳 稳定性 防腐剂 forensic toxicological analysis carbon monoxide stability perservatives
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