摘要
目的:研究不同温度处理对川贝母生理指标及鳞茎产量和总生物碱含量等的影响,为川贝母的合理栽培生产提供一定理论依据。方法:用人工气候箱控制川贝母的生长温度,观察不同温度15,20,25,30℃下川贝母的生长发育变化,检测其叶片叶绿素a和b、可溶性糖、丙二醛和脯氨酸含量,并测定鳞茎产量以及总生物碱含量。结果:15,20℃处理的川贝母生育期较长;4种温度处理下川贝母叶绿素b含量差异均不显著,且15,20,30℃下川贝母总叶绿素以及叶绿素a含量差异也不显著。此外,川贝母叶片可溶性糖、丙二醛、脯氨酸和鳞茎总生物碱含量,以及鳞茎增长率和干重均随温度降低而增加。结论:川贝母不宜生长在过高温度环境,相对较低温度下其生育期较长,鳞茎可适当生长,生物碱含量也有所增加。此外,低温下川贝母通过增加脯氨酸以及可溶性糖含量等来提高抗寒性,并能维持正常的叶绿素含量。
Objective ： To study the physiologic indexes, yield and the contents of alkaloids of Fritillaria cirrhosa D. Don un- der different temperature. Method- The growth temperatures （ 15, 20, 25, 30℃ ） of F. cirrhosa were controlled by using artificial climate, the growth was observed, the contents of chlorophyll a and b, soluble sugar, MAD, proline of the leaves of F. cirrhosa were tested, and the yield and the alkaloids content of the bulbs were analyzed. Result： The growth period of F. cirrhosa under 15,20 were appropriately extended. The difference of the content of leaves chlorophyll b under four temperatures and the contents of total chlo- rophyll and chlorophyll a under 15,20, 30 ℃ were not significant.. The contents of soluble sugar, MAD and proline of leaves and the growth ratio, dry weight and content of alkaloids of bulb increased with the temperature decrease. Conclusion： Higher temperature is not suitable for the growth of F. cirrhosa. Under the relatively lower temperature, the growth period of F. cirrhosa extended, the bulb can grow properly, and the content of alkaloid increased. F. cirrhosa can improve its cold tolerance by increasing the content of proline and soluble sugar, and it also can maintain the normal content of chlorophyll under the lower temperature.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第16期1948-1951,共4页
China Journal of Chinese Materia Medica
基金
国家“十一五”科技支撑计划(2006BAI06A13-1)
关键词
川贝母
温度
生理
鳞茎产量
总生物碱
Fritillaria cirrhosa
temperature
physiology
bulb yielding
total alkaloids
