摘要
研究了不同温度(8℃/5℃、15℃/12℃、22℃/15℃、28℃/25℃、35℃/28℃)处理对醉马草内生真菌共生体幼苗的形态指标、叶绿素、可溶性糖以及麦角酰胺和麦角新碱含量的影响,以期明确共生体幼苗生长和产碱的最适温度。结果表明,株高、根长和生物量均在22℃/15℃处理下达到最大,其中株高显著(P<0.05)高于其它4个处理,根长显著(P<0.05)高于28℃/25℃和35℃/28℃处理,生物量显著(P<0.05)高于8℃/5℃、28℃/25℃和35℃/28℃处理,分蘖在15℃/12℃处达到最大,显著(P<0.05)高于8℃/5℃、28℃/25℃和35℃/28℃处理。叶绿素在22℃/15℃处理下达到最大,显著(P<0.05)高于8℃/5℃、15℃/12℃和35℃/28℃处理。可溶性糖在5个温度处理下差异不显著(P>0.05)。在处理时间为15 d时,麦角酰胺和麦角新碱含量均在22℃/15℃处理下达到最大。综上所述,最适宜共生体幼苗生长、麦角酰胺和麦角新碱积累的处理温度是22℃/15℃。
study was conducted to investigate the effects of different alternate temperatures（ 8 ℃ /5 ℃,15 ℃ /12 ℃,22 ℃ /15 ℃,28 ℃ /25 ℃,35 ℃ /28 ℃） on variations of growth and ergot alkaloids concentrations of Achnatherum inebrians associated with Epichloё sp seedling. The results showed that the plant height,root length and biomass of A.inebrians had peak value under 22 ℃ /15 ℃,and the plant height had significant differences（P〈0. 05） with the other four treatments; the root length with treatment of 22 ℃ /15 ℃ was significant higher（P〈0. 05） than that with treatments of 28 ℃ /28 ℃,25 ℃ and 35 ℃ /28 ℃; biomass with treatment of 22 ℃ /15 ℃ was significant（P〈0. 05）higher than that with treatments of 8 ℃ / 5 ℃,28 ℃ /25 ℃ and 35 ℃ / 28 ℃. The tiller number of A. inebrians had peak value under 15 ℃ /12 ℃,and was significantly（P〈0. 05） higher than that with treatment of 8 ℃ /5 ℃,28 ℃ /25℃ and 35 ℃ /28 ℃. Chlorophyll was maximum under treatment of 15 ℃ /22 ℃,and was significant higher（P〈0. 05）than that with treatments of 8 ℃ /5 ℃,15 ℃ /12 ℃ and 35 ℃ /28 ℃. Soluble sugar had no significant difference between these five temperature treatments. The contents of ergine and ergonovine had a peak value under 22 ℃ / 15 ℃ after 15 d,which had significant difference（P〈0. 05） with other treatments. In conclusion,the optimal temperature for seedling growth and the accumulation of ergine and ergonovine treatment was 22 ℃ /15 ℃.
出处
《草业科学》
CAS
CSCD
北大核心
2016年第7期1353-1360,共8页
Pratacultural Science
基金
国家“973”项目(2014CB138702)
国家自然科学基金项目(31372366)
教育部创新团队发展计划项目(IRT13019)
