摘要
目的探讨阿司匹林对兔动脉粥样硬化及其炎性过程的影响。方法18只雄性新西兰兔随机分为对照组(喂食普通兔饲料)、高脂模型组(喂食高脂饲料)、阿司匹林组(喂食高脂饲料并给予阿司匹林干预),饲养12周后处死动物,取主动脉进行病理学检查,采用免疫组化SP方法观察各组斑块区环氧合酶2(COX-2)的表达、巨噬细胞和平滑肌细胞的数量变化。结果病理学大体观察,对照组、高脂模型组和阿司匹林组斑块/内膜面积比分别为0、(59.6±13.7)%和(49.3±7.8)%,组间比较差异有极显著性意义(P<0.01);光镜下动脉粥样硬化斑块多参数分析显示,斑块最大厚度、管腔狭窄度和斑块所占周径比值3个指标各组间两两比较差异均有极显著性意义(P<0.01)。免疫组化检测结果显示:阿司匹林组斑块区COX-2表达、巨噬细胞数量明显低于高脂模型组,平滑肌细胞增殖和迁移也低于高脂模型组。结论阿司匹林能明显减轻高脂饮食所致的动脉粥样硬化大小及程度。抑制斑块内COX-2的表达以及后续的炎症过程可能是其抗动脉粥样硬化的机制之一。
Objective To examine the effects of aspirin on atherosclerosis and its inflammation process in atherosclerotic rabbits. Methods Eighteen male New Zealand rabbits were randomly divided into 3 groups： control group, untreated cholesterol-fed group, aspirin-treated group, which were fed for 12 weeks. After 12 weeks, the aorta was harvested for pathologic morphology observation. By using immunohistochemistry, the expression of COX-2 and the changes in number of macrophages and vascular smooth muscle cells （VSMCs） were observed. Results The aorta plaque/intima size （P/I） was 0, （59.6±13.7）% and （49.3±7.8）% in control group, untreated cholesterol-fed group and aspirin-treated group respectively by pathologic morphology observation （P〈0.01）. The maximum plaque thickness, the degree of artery stenosis and the proportion of the intimal circumference occupied by atheroma in 3 groups were different from each other significantly （P〈0.01）. The expression of COX-2, the number of macrophages and the VSMCs proliferation and migration from media to intima in the plaque of aspirin-treated group were decreased as compared with those in untreated cholesterol-fed group. Conclusion Aspirin can suppress the progression of experimental atherosclerosis and stabilize the plaque. The expression of COX-2 in plaque was also decreased after aspirin treatment, which may be one of the mechanisms by which aspirin suppresses the progression of experimental atherosclerosis.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2008年第4期486-489,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
