摘要
目的:建立与穿心莲的生物有效性相关的HPLC指纹图谱。方法:采用HPLC对穿心莲及其代谢产物进行研究,以乙腈-0.1%甲酸水进行梯度洗脱,柱温为25℃,流速为1.0mL/min,检测波长为254nm。收集灌流液进行LC /MS分析,采用ESI离子源、二级串联质谱、负离子检测方式进行检测。结果:建立了精密度、稳定性和重复性均良好的HPLC指纹图谱检测方法,并对该指纹图谱中被吸收的特征峰与成分进行定性与定量分析,从而制定与生物有效性相关联的穿心莲化学成分指纹图谱。结论:该方法的建立为今后同类药物的定性定量分析提供基础。
Objective： To develop a method of high performance liquid chromatography for the analysis of biological availability of Andrographis paniculata（Burm.f. ） Nees. Methods： Study on Andrographis paniculata（Burm.f.） Nees and their metabolites by HPLC, the mobile phase was acetonitrile- water（ 0.1% methanoic acid） for gradient elution, Column temperature was 25℃ , flow rate was 1.0 mL/min, the detection wavelength was at 254nm.. Collected the perfusate and analysed by LC/MS, MS： dectrospray ionization （ESI）, second contacts the mass spectrum, negative ions analysis. Results： To establish the fingerprints which has high precision and accuracy, Qualitative and quantitative analysis to characteristic peak of fingerprint which been absorbed. And to establish the fingerprint of Andrographis paniculata （Burm.f.） Nees Conclusions It can be used research of this kind ofdrugs in further.
出处
《黑龙江医药》
CAS
2008年第5期33-35,共3页
Heilongjiang Medicine journal
关键词
穿心莲
高效液相色谱法
生物模型表达
Andrographis paniculata（Burm.f.）
HPLC
fingerprint
Biological Expression
