摘要
利用云芝菌固态发酵产漆酶，当玉米皮和麸皮的比例为6：4，培养基含水量65％，接种量10％，培养温度30℃时，发酵10d，漆酶活力可达到1．51×10^6U／g（干曲）。利用云芝漆酶对2，4-二氯酚进行脱氯反应，其适宜温度为35℃，pH6．0，当底物浓度为1mmol／L时，反应8h，2，4-二氯酚的去除率达到95％以上。
The solid state fermentation process for laccase production was investigated using Coriolus versicolor. The maximal laccase activity reached 1.51 × 10^5 U/g for 10 d when the ratio of the concentration of corn husk were 65 formed by to bran was 6 ： 4 and the water content of medium, inoculation volume, fermentation temperature , 10% and 30 ℃, respectively. The dechlorination of 2, 4-dichlorophenol in wastewater was performed by the laccase in a reactor. The optimal temperature and pH value for the reaction were 35 ℃ and 6.0, respectively. The decholorination efficiency of laccase on 2, 4-dichlorophenol （1 mmol/L） was above 95%.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第6期32-36,共5页
Food and Fermentation Industries
关键词
漆酶
固态发酵
云芝菌
2
4-二氯酚
脱氯
废水
solid state fermentation, Coriolus vericolor, 2, 4-dichlorophenol, decholorination, wastewater
