摘要
利用29个培养基配方,进行了云芝菌液体摇瓶发酵试验,考察了发酵菌粉的多糖质量分数和菌粉干质量。结果表明:以菌粉多糖质量分数不低于0.04,菌粉干质量不低于20 g/L为标准,最优培养基配方为黄豆饼粉20 g/L,葡萄糖30 g/L,玉米粉10 g/L,酵母膏5 g/L,Ca CO31.5 g/L,KH2PO41.0 g/L,Mg SO41.5 g/L,p H自然,多糖质量分数为0.049 4;菌体干质量为21.00 g/L。
29 mediums were applied to produce mycelium by liquid fermentation of Coriolus versicolor,and the intracellular polysaccharide contents and dry cell weights of mycelium under different mediums were evaluated respectively. When intracellular polysaccharide mass fraction（ more than 0. 004 0） and cell dry weight（ more than 20 g/L） were achieved,No. 14 was chosen as a suitable medium.
出处
《河北科技师范学院学报》
CAS
2015年第1期34-38,共5页
Journal of Hebei Normal University of Science & Technology
基金
国家"863"计划项目(项目编号:2012AA02170)
2013年江苏省科技厅企业"博士集聚"计划和江苏省农业科技支撑项目(项目编号:BE2012341)
关键词
云芝
液体发酵
培养基
Coriolus versicolor
liquid fermentation
mediums
