摘要
目的探讨转化生长因子β1及其信号转导分子Smad3在肌成纤维细胞C2C12殖中的作用。方法以转化生长因子β1作用于C2C12细胞,对其信号转导分子Smad3基因进行RNA干扰,用MTT法检测转化生长因子β1诱导的C2C12细胞增殖。结果0、1、5和10μg/L转化生长因子β1作用C2C12细胞24h后,C2C12细胞增殖随转化生长因子β1浓度增加而增加,且呈剂量依赖性(光密度值分别为0.096±0.015、0.177±0.014、0.240±0.028和0.312±0.012,P<0.01);经200pmol/LsiRNA-Smad3转染24h后,再用5μg/L转化生长因子β1作用,其细胞增殖(光密度值0.063±0.011)比内对照组下降(光密度值0.137±0.016,P<0.01)。结论转化生长因子β1通过Smad3信号转导促进C2C12细胞增殖并呈剂量依赖性,siRNA-Smad3可有效阻断其信号转导而降低C2C12细胞增殖。
Aim To investigate the effects of Smad3 on the growth of C2C12 cells induced by transforming growth factor-β1 （TGF-β1 ）. Methods C2C12 cells were transfected with siRNA-Smad3 in vitro, and the cell proliferations were examined with MTT methods. Results After the C2Cl2 cells interfered with different concentrations of TGF-131 （0, 1, 5, 10μg/L） for 24 h, the MTT （OD） values were 0.096± 0.015, 0.177±0.014, 0.240±0.028, 0.312±0.012 respectively （P〈0.01）. While the C2C12 cells were transfected with 200 pmol/L siRNA-Smad3 for 24 h and then treated with 5 μg/L of 3GF-β1, the MTT （ OD ） values in experimental group （ 0. 063 ± 0.011 ） were decreased compared with internal control group （ 0.137 ± 0. 016, P 〈 0.01 ）. Conelusion The TGF-β1 could promote C2Cl2 cells proliferation by the Smad3 pathway and showed dose dependent manner, siRNA-Smad3 could block the signal transduction and decreased C2Cl2 cell proliferation effectively.
出处
《中国动脉硬化杂志》
CAS
CSCD
2008年第4期281-283,共3页
Chinese Journal of Arteriosclerosis
基金
湖南省卫生厅科研基金(B2005-072)
教育部留学回国启动基金(2006-331)
