摘要
用化学发光杂交试剂代替３２Ｐ进行菌落原位筛选重组Ｐ５３阳性克隆，可减少同位素的放射性损害和污染及半衰期短等缺点．本方法实验周期短、特异性可和同位素相当，可用于重组目的基因的原位杂交筛选．
Enhanced chemiluminescent(ECL) reagent instead of 32 P isotope was used in the screening of bacterial colonies which contain recombinant P53 cDNA.It overcomes the disadvantages of radioactive injuries,pollution and shorter half time of 32 P isotope.Compared with radioautography,enhanced chemiluminescent autophotography needs less time and has the same specificity with the former.Therefore,it can be used conveniently in the screening of interested bacterial colonies.
基金
国家自然科学基金
关键词
菌落
克隆
原位杂交
化学发光
HRP DNA probe, In situ hybridization,PCR,Enhanced chemiluminescence
