摘要
介绍了一种改进的利用减法杂交构建λ噬菌体ｃＤＮＡ文库的方法，该方法利用处理的ｓｃＤＮＡ减去两种对照的ｍＲＮＡ富集目的ｓｓｃＤＮＡ并用磁珠法分离杂交单体，结果表明其增加了杂交单体的产量、简化了实验步骤，而且更有利于富集所需基因，是构建ｃＤＮＡ文库的一个有效改进．
An improved method was utilized for constructing hybrid arrested λ phage cDNA library by subtractive hybridization, in which magnetic particles were applied to separate monomers from hybrids, and candidate sscDNAs were enriched by subtracting two control mRNAs from one treated sscDNA. The results showed that this new approach had a efficient improvement in constructing cDNA library which increased the yield of monomers, simplified experimental procedures and enriched more candidate genes.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第4期373-376,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
关键词
CDNA文库
磁珠
减法杂交
构建
cDNA library, magnetic particle, subtractive hybridization
