摘要
目的：观察HFRS沙鼠肾灭活疫苗（Ⅰ型）加强免疫后的血清学效果及防病效果。方法：采用间接免疫荧光试验和双夹心ELISA法，检测血清中的IgG抗体；以微量细胞病变中和试验，检测血清中的中和抗体。结果：加强免疫前，血清抗体阳性率仅为18．3％，中和抗体阳性率为0％；加强免疫后2周，抗体阳性率升至90.1％，中和抗体阳性率达75％。加强免疫后1年内，接种组无1例病人发生，而未接种者发病5例。
Obective: The purpose of this report was to observe the serofogic and preventive effect of TyPe Ⅰinactivated vaccines against HFRS (prepared from tissue culture of Mongoian gerbils kidney)after rein forcement. Methods: Indirect immunofluorescent antibody methed and Enzyme-linked Immunosorbent Assay indirect sandwich method were used in testing IgG antibody in sera. Microcytopathic effect neutralization test was used in the detection of neutralizing antithey in sera. Results: Before reinforcement, the Positive rate of antithey in sera was 18. 3 %, and that of neutralization antithey was 0 %.2 weeks after reinforcement, the POsitive rate of antibody in sera was 90. 1 %, and that of nentralization antibody was 75 %. 1 year after reinforcernent, there was no patient found in the inoculation group, and there were 5 patients found in the control group.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1997年第5期342-344,共3页
Chinese Journal of Vector Biology and Control
关键词
肾综合征出血热
灭活疫苗
流行病学效果
Hemorhagic Fever with Renal Syndrome (HFRS)
Inactivated vaccine reinforced immunization
On-the-spot experiment
