摘要
目的:研究葡萄籽提取物原花青素(procyanidins,PC)舒张家兔离体主动脉和降低其动脉血压的作用及机制。方法:采用家兔离体胸主动脉环灌流模型,将标本分6组:去内皮、内皮完整、吲哚美辛(1×10^(-1)mol·L^(-1))、普萘洛尔(1×10^(-5)mol·L^(-1))、左旋硝基精氨酸N~ω-nitro-L-arginine(L-NNA 1×10^(-4)mol·L^(-1))或甲烯蓝(MB 1×10^(-5)mol·L^(-1)),分别累积加入1.25,2.5,5.0 mg·L^(-1)PC观察血管张力变化;并观察40 mg·L^(-1)PC对去甲肾上腺素(NA)(1×10^(-8)～1×10^(-5)mol·L^(-1))、KCl(6.3～100 mmol·L^(-1))和CaCl_2(1×10^(-5)～1×10^(-2)mol·L^(-1))诱导血管环收缩曲线的影响。另用家兔颈总动脉插管法,经静脉累积注射4.0,8.0,16,32,64,84 mg·kg^(-1)PC后观察血压变化。结果:PC能舒张主动脉血管,并有量效依赖性(r=0.63,P<0.001),去内皮、L-NNA或MB可减弱PC的舒张作用。PC能抑制NA,KCl和CaCl_2的量效收缩反应。PC能降低家兔正常动脉血压并有量效依赖性(r= 0.92,P<0.001)。结论:PC舒张血管的作用是通过抑制细胞内Ca^(2+)释放和电压依赖性Ca^(2+)通道而减少Ca^(2+)内流;也与内皮释放的NO有关;PC可降低家兔正常的动脉血压。
Objective： To study the vasodilation effect of the procyanidin （PC） extracted from grape seeds on rabbit thoracic aortic rings in vitro, decreasing blood pressure in vivo and the possible mechanism. Method：. Rabbits aortic rings were isolated and were divided into six groups including removal of endothelium, integrity of endothelium, 1×10^-5 mol·L^-1 indomethaein （Indo） , 1×10^-5 tool·L^-l propranolol （Prop）, 1×10^-4 mol·L^-1 N^w-nitro-L-arginine （L-NNA） and 1×10^-5 mol·L^-l methylene blue （MB）. Then the thoracic aortic rings were treated with PC with cumulative concentrations of 1.25, 2. 5, 5.0 mg·L^-1 respectively and the changes of tension were recorded, and investigate the effect of 40 mg·L^-1 PC on the contraction of aortic smooth muscles, thoracic aortic rings were pre-treated with NA （1×10^-8 - 1×10^-5 mol·L^-1 ）, KCI（6.3-100 mmol·L^-1） and CaCl2（1×10^-5 - 1×10^-2 mol· L^-1） followed by treatment with PC. Then, rabbits common carotid artery was intubated and arterial blood pressure in vivo was recorded. PC with cumulative concentrations of 4. 0, 8. 0, 16, 32, 64, 84 mg·kg^-1 was injected into vein and the changes of arterial blood pressure were observed. Result： PC could relax isolated rabbit aorta and showedan obvious concentration-dependent relaxation （r=0.63, P〈0.001）. The relaxant effect of PC was significantly reduced by removal of endothelium and by treatment with nitric oxide synthase inhibitor L-NNA, or guanylyl eyelase inhibitor MB. In addition PC could decrease the dose response curves of aortic rings to NA, KCl and CaCl2, PC has a significant concentration-dependent negative effect on arterial blood pressure in vivo （r=0.92, P〈0.001）. Conclusion： PC has a vasodilation effect not only in an endothelium-dependent, nitric oxide involved manner, but in inhibition of calcium release and blockage of potential-dependent calcium channels. PC could decrease the rabbit＇s arterial blood pressure significantly in vivo.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第14期1720-1723,共4页
China Journal of Chinese Materia Medica
关键词
原花青素
离体主动脉
内皮细胞
钙离子
动脉血压
proeyanidin
isolated aortic ring
endotheliumz
Ca^2＋
arterial blood pressure
