摘要
目的探讨高脂饮食对大鼠脂肪肝胰岛素受体底物(IRS)1、IRS-2分子表达的影响。方法对8周高脂饲养(n=7)和正常饲养(n=7)大鼠的胰岛素敏感性、脂质代谢、肿瘤坏死因子(TNF)α以及肝脏IRS-1、IRS-2的mRNA和蛋白表达进行检测。结果与正常组比,高脂组大鼠呈现胰岛素抵抗、游离脂肪酸(FFA)和TNF-α增高;肝脏的IRS-1 mRNA和蛋白含量分别降低了28%和32%(P<0.01),IRS-2 mR-NA和蛋白含量分别降低了30%和27%(P<0.01)。结论高脂饮食导致大鼠肝脏IRS-1、IRS-2的mR-NA和蛋白含量明显降低,这可能是高脂饮食引起脂肪变肝脏胰岛素抵抗的重要分子机制。
Objective To explore the effect of high-fat diet on IRS-1 and IRS-2 expressions of fatty liver in rat. Methods After the rats were fed a high-fat diet （n=7） and a standard chow（n=7） for 8 weeks, insulin sensitivity,lipid metabolism, TNF-α and hepatic mRNA and protein expressions of IRS-1 and IRS-2 were measured. Results Compared with the normal control, the high-fat diet group displayed the elevated levels of insulin resistance,FFA and TNF-α. Hepatic IRS-1 mRNA expression and IRS-1 protein content were reduced by 28 % and 32% （P〈0. 01）,respectively. Similarly, hepatic IRS-2 mRNA and IRS-2 protein content were also reduced by 30% and 27% （P〈0.01）, respectively. Conclusions High-fat diet results in a significant decrease in expressions of mRNA and protein of hepatic IRS-1 and IRS-2 in rats, which might be one of the molecular mechanisms of insulin resistance in liver steatosis induced by high-fat diet.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2008年第7期409-412,共4页
Chinese Journal of Diabetes
关键词
高脂饮食
胰岛素抵抗
胰岛素受体底物
High-fat diet
Insulin resistance
Insulin receptor substrate
