摘要
目的:研究邻苯二甲酸二丁酯(DBP)对雌性果蝇生殖能力的影响。方法:将未交配雌性果蝇随机分为5组,分别给予含DBP0.1%(A组)、0.4%(B组)、1.6%(C组)和6.4%(D组)的培养基喂饲,并以0浓度为对照组(E组),在染毒10d和20d时,分别取存活的实验雌蝇进行交配试验和生育试验。结果:各染毒组雌蝇1h交配率随DBP浓度升高而降低,D组雌蝇的1h交配率和生育率明显低于E组(P<0.05或P<0.01);染毒10d后C组的平均生育数和平均产卵期均明显高于E组(P<0.05),且在连续产卵10d后仍保持较高的生育水平;染毒20d后D组的平均生育数和平均产卵期均明显低于E组(P<0.01)。结论:DBP对雌蝇生殖能力有双重作用,较低浓度可提高雌性果蝇生育力,而较高浓度可使其生育能力降低。
Objective： To explore the effects of di-n-butyl phthalate （DBP） on the procreative ability in female Drosophila melanogaster. Methods： The non-copulated female flies were divided randomly into 5 groups and were continuously fed with medium containing 0.1% （group A）, 0.4% （group B）, 1.6% （group C）, 6.4% （group D） DBP, and with mdium containing 0 DBP as the control （group E）. The treated female flies were brought to the copulation trial and the reproduction trial on exposure d 10 and d 20, respectively. Results： During 1 h the mating rates of female flies in DBP groups were gradually decreased with the rising of DBP concentration. The mating rate in 1 h and the reproduction rate of female flies in group D were lower observably than those in group E （P〈0.05 or P〈0.01）. After exposure 10 d, the average reproduction quantity and the average procreant period of female flies in group C were observably higher than those in group E （P〈0.05）. There was still higher reproduction quantity in group Catter continuously ovipositing 10 d. After exposure 20 d, the average reproductive quantity and the average procreant period of female flies in group D were observably lower than those in group E （P〈0.01）. Conclusion： DBP might bring out the two-sided effects on the procreative ability of female Drosophila melanogaster. DBP in lower concentration might enhance the procreative ability of female flies, but reduce that while DBP in highter concentration.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2008年第6期329-333,共5页
Reproduction and Contraception
基金
上海市卫生局科研基金项目
项目号:054092
