摘要
目的探讨DNA修复基因x线修复交叉互补因子3（X-ray cross—complementing group 3，XRCC3）基因Thr241Met多态性与黄曲霉毒素B1（aflatoxin B1，AFB1）相关性肝细胞癌（hepatocellular carcinoma，HCC）遗传易感性的相关性。方法应用PCR技术对AFB1高污染区广西地区257例HCC患者和711名对照人群的XRCC3基因多态性进行检测，进行的病例对照研究。结果（1）XRCC33种基因型（Thr／Thr、Thr／Met、Met／Met）中带有Met者与HCC的易感性相关，且这种相关性与Met数量呈正相关（校正风险值OR分别为2．20和8．56）；（2）XRCC3突变基因型多态与血白细胞AFB1-DNA加合物水平在HCC发生过程中存在协同作用（校正OR：2．34～20．44，P〈0．01）。结论XRCC3多态性与HCC易感性相关，且这种多态性与AFB1暴露水平在HCC发生中存在协同作用。
Objective To explore the association of the Thr241Met polymorphism of X-ray cross-complementing group 3 （XRCC3）gene with genetic susceptibihty to aflatoxin BI（AFB1 ）-related hepatocellular carcinoma （HCC）in Guangxi population. Methods We conducted a hospital-based case-control study, including 257 HCC cases and 711 controls without cancers or liver diseases. The XRCC3 Thr241Met polymorphism was analyzed by PCR. Results The XRCC3 genotypes XRCC3-Thr/Met or XRCC3-Met/Met were related with an elevated risk of HCC. The risk of HCC was associated with the number of mutant Met copies （adjusted OR were 2.20 and 8.56 for XRCC3-Thr/Met and Met/Met, respectively）; moreover, there seemed to be combined effects for HCC risk between the variant genotypes and AFB1- DNA adduct levels from peripheral blood leukocytes （ adjusted OR was 2.34 to 20.44, P 〈 0.01 ）. Conclusion These results suggested that XRCC3 polymorphism may be associated with the risk of AFB1-related HCC among the Guangxi population, and interacts with AFBl exposure in the development of HCC induced by AFB1 .
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2008年第3期268-271,共4页
Chinese Journal of Medical Genetics
基金
右江民族医学院自然科学基金（2005和2007）,国家自然科学基金（39860032）
