摘要
目的通过研究潜在抑癌基因KLF6在肝细胞癌的表达,探讨KLF6基因作为肝癌抑制基因的可能性。方法分别用荧光定量PCR、半定量RT-PCR和Western blot方法检测肝细胞癌及癌旁组织中KLF6基因在mRNA及蛋白水平的表达变化。结果实时荧光定量PCR显示经过看家基因GAPDH标化的KLF6 cDNA起始浓度,在配对癌旁组织约为肝癌组织的3倍。26例肝癌组织的KLF6 mRNA表达水平与配对癌旁组织相比,差异具有显著性意义(P<0.01)。在肝癌细胞株HepG2及Hep3B,KLF6 mRNA的表达亦显著低于正常对照细胞L02(P<0.01)。实验还发现,在肝细胞癌中KLF6蛋白表达变化与mRNA表达变化一致,即KLF6蛋白在癌组织及肝癌细胞的表达明显低于癌旁组织及L02(P<0.01)。结论KLF6表达下调可能是肝细胞癌的遗传学改变之一,KLF6基因可能为新的肝癌相关抑癌基因。
Objective To investigate the expression change of KLF6 gene in hepatocellular carcinoma （HCC） and the possibility of KLF6 gene as a tumor suppressor gene for HCC. Methods Fluorescent quantitative PCR, semi-quantitative RT-PCR and Western blotting were used for the detection of the expression of KLF6 mRNA and protein in HCC tissues, adjacent normal tissues and hepatoma cell lines （Hep3B and HepG2）. Results Fluorescent quantitative PCR showed that the concentration of KLF6 cDNA in adjacent normal tissues, normalized by the concentration of housekeeping gene GAPDH, was three times of that in HCC tissues. KLF6 mRNA level was significantly decreased in HCC tissues as compared with that in adjacent tissues （t = 3. 683., P〈0.01）. KLF6 mRNA level in hepatoma cell lines Hep3B and HepG2 was substantially lower than that in normal control cell line L02 （P〈0.01）. Moreover, the expression change of KLF6 protein was in line with that of KLF6 mRNA. Significant differences were found in the expression of KLF6 mRNA and protein between HCC tissues and adjacent tissues and among Hep3B, HepG2 and L02 （P〈0. 01）. Conclusion The down-regulation of KLF6 gene may be involved in the genetic changes of HCC. KLF6 gene is expected to be a new HCC-associated tumor suppressor gene.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2008年第3期343-346,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
KLF6
肝细胞癌
基因表达
Kruppel-like factor 6
hepatocellular carcinoma, gene expression
