摘要
目的:探讨野生型抑癌基因KLF6对前列腺癌LN-CaP细胞的生长增殖、细胞周期和侵袭转移能力的影响及其可能的作用机制.方法:利用RT-PCR法克隆目的基因KLF6,并将含KLF6的pEGFP-C1质粒转染入LNCaP细胞.分为转染组和对照组分别进行MTT法观察LNCaP细胞的生长抑制率,流式细胞仪观察细胞周期比例变化和凋亡率,细胞爬片划痕法观察LNCaP细胞的侵袭转移能力变化.结果:转染了野生型抑癌基因KLF6的前列腺癌LNCaP细胞生长抑制率为(31.9±4.7)%,对照组为0%,差异有统计学意义(P<0.01),细胞周期比例表现为G2/M期减少,G0/G1期比例增加为(75.0±8.8)%,对照组为(55.9±7.1)%,差异有统计学意义(P<0.05),细胞凋亡峰为(29.3±3.7)%,对照组为(8.6±0.9)%,差异有统计学意义(P<0.01);每毫米划痕区内迁移入的细胞数为102.8±15.4,对照组为(192.7±25.2),差异有统计学意义(P<0.05).结论:野生型抑癌基因KLF6的转染可以明显抑制前列腺癌LNCaP细胞的生长增殖,并诱导其调亡,使其侵袭转移能力下降,其机制可能与野生型抑癌基因KLF6部分地逆转LNCaP细胞的恶性表型有关.
AIM： To observe the effect of wild type -KLF6（wt- KLF6） on proliferation,cell cycle,invasion and migration of prostate cancer cell line LNCaP by transgenic method. METHODS： Wt-KLF6 cDNA was obtained by RT-PCR method from liver cell and inserted into plasmid pEGFP-C1 for transfecting into LNCaP cells. The untransfected LNCaP cells served as the controls. MTT was used for observing the cell growth suppression, flow cytometer for apoptosis and cycle phase, invasion capability experiment for cell invasion capability with anti-oncogene wt-KLF6 on prostate cancer cell line LNCaP by transgenic method for 48 h. RESULTS： After wt-KLF6 was transfected into LNCaP cells, the growth suppression of LNCaP cells was enhanced [ （31. 9 ± 4.7 ） % vs 0%, P 〈 0.01 ], the apoptosis was increased [ （ 29.3 23.7）% vs （8.6 20.9）% , P 〈0.01] , the invasion capability was decreased[ immigrated cell population/mm^2, （ 102. 8 ± 15.4） vs （ 192.7 ± 25.2）, P 〈 0.05 ]. Wt-KLF6 also decreased the ratio of LNCaP cell in phase G2/M, increased ratio of G0/G1 from control group （ 55.9 ± 7. 1 ） % to transfection group （75.0 ± 8.8） % （ P 〈 0. 05 ）. CONCLUSION ： Wt-KLF6 transfection can suppress the growth and induce the apoptosis, decrease the invasive capability of LNCaP cells, probably by reversing partially its malignant phenotype.
出处
《第四军医大学学报》
北大核心
2007年第14期1257-1259,共3页
Journal of the Fourth Military Medical University
