摘要
目的:检测食管鳞状细胞癌(ESCC)组织中错配修复基因hMLH1、hMSH2及P53的表达。方法:采用免疫组织化学SP法检测62例ESCC组织、31例癌旁不典型增生组织及62例正常食管黏膜组织中hMLH1、hMSH2及P53蛋白的表达。结果:ESCC组织、癌旁不典型增生组织与正常食管黏膜组织中hMLH1的阳性表达率分别为27.4%,58.1%及88.7%,hMSH2的阳性表达率分别为32.3%,51.6%及91.9%,P53的阳性表达率分别为79.0%,54.8%及35.5%,3种组织中hMLH1、hMSH2及P53的阳性表达率差异均有统计学意义(P<0.05)。ESCC组织中hMLH1、hMSH2及P53的表达与分化程度、浸润深度及有无淋巴结转移有关(P均<0.05);hMLH1、hMSH2阳性表达者P53的阳性率均低于hMLH1、hMSH2阴性表达者。结论:ESCC组织中存在hMLH1与hMSH2的低表达及P53的高表达,3者可能在食管癌的浸润、转移及黏膜上皮癌变过程中起重要作用。
Aim ： To investigate the expression of human mismatch repair gene（hMLH1/hMSH2） and P53 in esophageal squamous cell carcinoma （ESCC） tissue and its significance. Methods：SP immunohistochemieal method was used to detect the expression of hMLH1 ,hMSH2 and P53 protein in 62 cases of ESCC specimens, 31 cases of adjacent atypical hyper- plasia epithelium and 62 cases of normal esophageal epithelium. Results： The positive expression rates of hMLH1 in ES- CC, adjacent atypical hyperplasia epithelium and normal esophageal epithelium were 27.4% , 58. 1% and 88.7% , and those of hMSH2 were 32.3% , 51.6 % and 91.9% , and those of P53 were 79.0% ,54.8% and 35.5% , respectively. The expression of hMLH1, hMSH2 and P53 protein was associated with the differentiation levels, depth of invasion and lymph node metastasis of ESCC （P 〈 0.05）. The expression rate of P53 in cases with negative expression of hMLH1 and hMSH2 was higher than those with positive expression of hMLH1 and hMSH2. Conclusion ： hMLH1, hMSH2 and P53 play important roles in the infiltration, metastasis and carcinomatous change of the mucosa epithelium in ESCC.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2008年第3期412-414,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关计划基金资助项目0624410091
