摘要
目的探讨慢性丙型肝炎病毒(HCV)患者体内准种的多样性及动态变化过程与病程进展的关系。方法应用异源双链泳动技术(HMA)研究在干扰素治疗压力下慢性HCV患者体内准种的变化。结果慢性HCV患者无论对干扰素应答情况如何,其体内HCV准种的数量无明显变化;治疗前占支配地位的准种株群在不同感染者体内的比例并不相同,随着抗病毒治疗的进行,不断有新的异质性程度更高的准种株出现,打破原有的平衡比例。结论慢性HCV患者对干扰素治疗有无应答,取决于变异株的异质性程度,而不是变异株数量。
OBJECTIVE To investigate the quasispecies diversity and the dynamics of quasispecies evolution in patients with chronic Hepatitis C virus （HCV） infection. METHODS Changes of HCV quasispecies before and after interferon （IFN） therapy were detected by heteroduplex mobility analysis （HMA） and by automatic sequencer. RESULTS We found that no changes in viral complexity were observed with therapy, but there were marked changes in viral divergence. The proportion of major pretreatment variants was different in HVR1 in all patients. During the treatment, HCV quasispecies changed significantly. CONCLUSIONS HMA is a simple, rapid and reliable method for detecting HCV quasispecies. Responsiveness to interferon may be related to the changes in viral divergence, but not related to the numbers of viral variants.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第6期763-765,共3页
Chinese Journal of Nosocomiology
基金
黑龙江省自然科学基金资助(D2004-47)
