摘要
以黑曲霉为菌株,以农产品加工副产物为主要物料,采用固态发酵方法生产果胶酶,优化发酵培养基主要物料和培养条件。实验结果表明:培养基的主要物料为麸皮与豆粕,其比例为8∶2,加入3.5%的硫酸铵,培养基初始pH自然,250 mL三角瓶中适宜装料量为15 g,料水比为1∶1.5,接种量为106个孢子/g干基,在此优化条件下,果胶酶活力可以达到2262 U/g。
Pectinase was produced by Aspergillus niger through solid fermentation. By-products of agriculture processing products were applied as major materials. The results showed that the suitable culture medium within the scope of experiment was composed of bran and soybean meal （ratio of 8 to 2）, 3.5% ammonium sulfate. Initial pH of medium was nature. The optimized fermentation condition was that 15 g medium was packed in 250 mL flask, ratio of water and medium material was 1：1.5, the inoculum size was 108 spores per gram mediun. Activity of pectinase produced on optimized condition reached 2262 U/g per gram medium.
出处
《食品科技》
CAS
北大核心
2008年第5期14-18,共5页
Food Science and Technology
基金
黑龙江省自然基金项目(C2004-34)
教育部科学技术研究重点项目(202038)
关键词
果胶酶
黑曲霉
固态发酵
pectinase
Aspergillus niger
solid fermentation
