摘要
利用三重融合PCR方法构建了小鼠prnp基因敲除载体。提取129小鼠基因组,设计引物扩增打靶载体同源臂,把两同源臂与neo基因三元件PCR扩增成一条融合基因。融合基因与AAV载体酶切后的载体骨架通过NotI酶切位点相连接,构建重组质粒载体rAAV。
The adeno- associated virus vector for mouse prnp gene targeting was constructed by using three -way fusion PCR method. 129 mouse genomes were extracted, the primer for amplification of homology arms was designed, and then the left homology arm, right homology arm and neo gene were fused to one gene. The recombination plasmid vector rAAV was constructed by ligating fusion gene into AAV vector backbone through Notl restriction enzyme site.
出处
《江西农业学报》
CAS
2008年第5期78-81,共4页
Acta Agriculturae Jiangxi
