摘要
对衍生于常用载体pB I 121的4种表达载体所获得的马铃薯转基因株系进行了PCR骨架整合分析,结果表明,左边界序列和短片段骨架基因更容易整合进基因组中,转入基因组的载体骨架片段的大小也不一样.这说明在转基因植株的检测中要注意骨架载体是否转入,以便消除生物安全性问题.
The PCR method is employed to analyze transgenic potato lines transformed by four different vectors that were derived from common vector pBI121. The results showed the sequences of LB and the shorter genes of vector backbone are integrated into potato genome easier and the size of vector backbone fragment integrated into genome of potato are different. The results demonstrate that it is necessary to detect transgenic lines in order to eliminate the problem about biological security.
出处
《海南大学学报（自然科学版）》
CAS
2006年第3期305-308,316,共5页
Natural Science Journal of Hainan University
关键词
转基因植株
载体骨架
整合
PCR
Transgenic lines
vector backbone
integration PCR
