摘要
目的探讨咖啡因在骨肉瘤细胞株(OS-U2)顺铂化疗中的增效作用。方法骨肉瘤细胞株经不同浓度(0.2、2、5、10、20μg/L)顺铂单药作用及分别添加0.2、2.0mmol/L咖啡因作用24、48、72h,通过MTT法检测各组细胞增殖情况,流式细胞术分析细胞凋亡水平,荧光显微镜下观察凋亡细胞的形态并记录其阳性率。结果在添加0.2、2.0mmol/L咖啡因的各浓度顺铂组中,骨肉瘤细胞增殖明显受抑,凋亡水平明显升高。咖啡因对顺铂杀伤骨肉瘤细胞株的增效作用存在量效及时效关系。结论咖啡因对骨肉瘤细胞株顺铂化疗具有显著增效作用,其机制可能是咖啡因能加强诱导骨肉瘤细胞凋亡,从而增强顺铂对骨肉瘤细胞株的杀伤作用。
Objective To explore the enhancement effect of caffeine in cisplatin-induced apoptosis of osteosarcoma cell line OS-U2. Methods The osteosarcoma cells were incubated with different concentrations of cisplatin （0.2, 2, 5, 10 and 20μg/L）, caffeine （0.2, 2.0mmol/L） and caffeine ＋ cisplatin for 24, 48 and 72 hours. The proliferation of OS-U2 cells was determined by MTT assay, the apoptotic levels were determined by flow cytometry （FCM）, and the morphologic changes of apoptotic cells and the positive rates of apoptosis were determined by fluorescence microscopy. Results The proliferation of OS-U2 cells was inhibited, and the apoptotic level was increased when incubated with caffeine and/or cisplatin. There were dose- and time-effect relationships between the lethal effect of cisplatin on osteosarcoma cells and caffeine. Conclusion There is a remarkable enhancement effect of caffeine on cisplatin-induced apoptosis of osteosarcoma cell line OS-U2. It seems that the apoptosis-inducing activity of caffeine may enhance the lethal effect of cisplatin on osteosarcoma cells.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2008年第5期582-583,592,共3页
Medical Journal of Chinese People's Liberation Army
基金
首都医学发展科学基金资助课题(2003-3029)
关键词
细胞凋亡
顺铂
咖啡因
骨肉瘤
apoptosis
cisplatin
caffeine
osteosarcoma
