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植物中的咖啡碱:从合成途径研究到转基因作物 被引量:3

Caffeine in Plants: From Biosynthesis Pathway to Genetically Modified
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摘要 在植物细胞中咖啡碱的核心合成途径为:黄嘌呤核苷→7-甲基黄嘌呤核苷→7-甲基黄嘌呤→可可碱→咖啡碱,即3次顺序甲基化反应和1次核糖水解过程。目前已经从茶树等植物中分离出催化上述甲基化反应的酶,并克隆得到编码这些酶的基因。在此基础上,利用RNAi干扰和农杆菌介导法等技术,已成功培育出低咖啡碱饮料作物茶树和咖啡以及能合成咖啡碱的烟草和菊花植株。 The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→ 7-methylxanthosine→ 7-methylxanthine → theobromine → caffeine. In recent years, the enzymes and genes involved in caffeine biosynthesis have been isolated from Camellia and Coffea plants and genetically modified tea and coffee seedlings that producing low caffeine have been developed. Caffeine can also be synthesized in transgenic tobacco and chrysanthemum plants, which do not contain it naturallv, for nests contrnl nnrnn~
出处 《园艺学报》 CAS CSCD 北大核心 2012年第9期1849-1858,共10页 Acta Horticulturae Sinica
基金 国家科技支撑计划项目(2009GJF00047) 四川省科技厅科技支撑计划项目(2006-YZGG-8,2009-NZ0015).现代农业产业技术体系建设专项资金项目(nycytx-23)
关键词 茶树 咖啡植物 咖啡碱 合成途径 N-甲基转移酶:转基因 Camellia sinensis Coffea caffeine biosynthesis N-methyltransferase geneticallymodified
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