摘要
目的探讨Fas、FasL及caspase-3凋亡相关蛋白在特发性血小板减少性紫癜（ITP）患者T淋巴细胞的表达及其意义。方法应用流式细胞术测定T细胞亚群表面Fas、FasL的表达率及细胞质中活化caspase-3的表达率，用Western blot法检测T细胞亚群caspase-3蛋白的表达。结果与健康对照组[（29.4±8.2）％]相比，ITP患者组CD4^＋T细胞表面Fas的表达率显著增加[（42.1±9．5）％]（P〈0．05），CD8^＋T细胞表面Fas的表达率略有增加但差异无统计学意义[（9．3±6．0）％与（13．4±5．8）％]（P〉0．05）。ITP患者组T细胞亚群表面FasL的表达率均较健康对照组显著增加（P〈0．05）。ITP患者组T细胞亚群胞质中活化caspase-3的表达率，明显高于健康对照组（P〈0．05）。Western blot检测结果显示，与治疗前相比，ITP患者治疗后CD4^＋T细胞表达pro-caspase-3和cleaved-caspase-3均明显减少（P〈0．05）。结论ITP患者外周血T细胞Fas、FasL及caspase-3表达明显增加，激素治疗可干预Fas—FasL、caspase-3的表达水平，提示Fas、FasL及caspase-3信号通路在ITP发生机制中起一定作用。
Objective To explore the relationship between Fas-FasL-mediated signal transduction pathway and apoptosis of T lymphocyte subset in ITP patients. Methods The expression rates of membrane Fas, FasL and intracellular activated caspase-3 in peripheral T lymphocyte subset were determined by flow cytometry. T cell subsets with caspase-3 protein expression were detected by Western blot. Results As compared with that in healthy control group [ （29.4 ± 8.2） % ] , the expression rate of membrane Fas on CD4^＋ T cells was significantly increased in ITP patients [ （42.1 ± 9.5） % ] （P 〈 0. 05） , however, that on CD8^＋ T cells was only slightly increased [ （ 9.3 ± 6.0） % vs （ 13.4 ± 5.8 ） % ] with no statistical significance （ P 〉 0. 05）. The expression rate of FasL on T cell subset in ITP patients was significantly increased （ P 〈 0. 05）, and that of intracellular activated caspase-3 in T cell subset in ITP patients was notably higher than that in healthy control group （ P 〈 0. 05 ）. Western blot analysis showed that the expression of pro-caspase-3 and cleaved-caspase-3 in CD4^＋ T cells in patients with ITP after treatment were significantly reduced compared with those before treatment （ P 〈 0. 05 ）. Conclusion Apoptosis of T lymphocyte subset in ITP patients is accelerated. It is possible that Fas-FasL signal transduction pathway plays an important role in the induction of the apoptosis. The degree of apoptosis of T lymphocytes closely correlates with the disease＇ s activity in ITP patients. Hormone therapy may interfere with Fas-FasL signal transduction pathway of apoptosis.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2008年第5期329-332,共4页
Chinese Journal of Hematology
