摘要
RP-HPLC分离三七粉提取液,并鉴定含有Rb1、Rg1、Re、R1等皂甙成分。以蓝斑背肛海兔(Notarcusleachii cirrosus Stimpson,NLCS)为分析模型,三七粉提取液为诱导剂,选用蛋白质组技术研究NLCS神经连索诱导前后所表达的差异蛋白质。通过优化双向凝胶电泳分离NLCS神经连索全蛋白质组技术,获得496个蛋白质斑点。采用肽指纹图谱技术和数据库检索比对法,初步鉴定了NLCS受三七粉提取液诱导前后,其神经连索表达13个差异蛋白质,其中较高的匹配率蛋白质为肌动蛋白、3-羟酯酰辅酶A脱氢酶、ATP结合转运子和甲基转移酶12。选用LOC tree软件对13个差异蛋白质进行亚细胞定位,认为它们在保护神经系统中发挥重要的调节作用。
Extract of Panax Notoginseng power was separated and its saponins component containing Rbl, Rgl, Re, R1 etc. was further identified by RP-HPLC approach synchronously. Using an analytical model of Aplysia （ Notarcus leachii cirrosus Stimpson, NLCS） and a revulsant of Panax notoginseng power, differential proteins of neural connective between control aplysia and experimental one were revealed by proteomic tech- niques in NLCS. Whole proteins of neural connective in aplysia were optimally separated by two-dimensional polyacrylamide gel electrophoresis （2D-PAGE）, obtaining approximately 496 protein spots. Thirteen differen- tial proteins of the neural connective between control aplysia and experimental one induced with Panax Notoginseng power were compared and identified by peptide mass fingerprinting （PMF） and databases searches, respectively. These differential proteins with high scores were primarily indicated to be Actin, 3-hydroxyacyl- CoA dehydrogenase, ABC transporter related, and methyhransferase type 12. In addition, these differential proteins vest further in its subcellular localization by LOCtree software.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2008年第5期577-582,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20776060)
福建省创新团队培养计划资助项目
关键词
三七
皂甙
差异蛋白质
分离
鉴定
蛋白质组技术
Panax notoginseng, saponins, differential protein, separation, identification, proteomics
