摘要
从蛋白质组学角度分析大鼠骨髓间充质干细胞(MSCs)体外定向分化为心肌细胞过程中蛋白表达情况,采用二维电泳分离蛋白,用PDQuest软件分析蛋白表达差异,并采用质谱(MALDI-TOF-MS)进行鉴定,得到了54个蛋白点,对蛋白的生物功能分析表明,部分蛋白通过不同的信号途径参与了MSCs的分化过程.
Bone marrow mesenchymal stem cells （MSCs） can be differentiated into cardiomyocytes with the induction of 5-azacytidine in vitro, and the induced cardiomyocytes can be used for cell transplantation for cardiac diseases. In the present study, the protein profiling of rat MSCs was investigated by two-dimensional gel electrophoresis（2DE）. According to the data analysis by PDQuest, 24 protein spots showed up-regulation, and 14 protein spots showed down-regulation during MSCs differentiation. However, there were 9 protein spots expressed only in undifferentiated MSCs, and 19 protein spots were detected in differentiated cells but not MSCs. Altered proteins were digested by trypsin and subjected to MALDI-TOF-MS analysis for peptide mass fingerprinting（PMF）. Fifty-four proteins were identified successfully from NCBInr and/or SWISS-PROT databases. Among the identified proteins, there were 20 proteins showed up-regulation, and 4 proteins down-regulated during the induced differentiation of MSCs. According to the previous reports of their function, these regulated proteins may be involved in the differentiation of rat MSCs. Our data suggested that proteomics can be used to highlight the study of molecular mechanism of cell differentiation.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第10期1881-1886,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:30271663
90209005和90209035)
国家'九七三'项目(批准号:2001CB510305和2005CB523503)资助.
关键词
骨髓间充质干细胞
蛋白质组学
分化
机理
Bone marrow mesenchymal stem cells（MSC）
Proteomics
Differentiation
Mechanism
