摘要
以纯化pQE31表达的融合蛋白仙台病毒FP(S)为诊断抗原、融合蛋白FP(S)免疫鼠血清为阳性抗体,建立了小鼠仙台病毒抗体检测的间接ELISA诊断方法。该抗原FP(S)不与其他常见的小鼠病毒(小鼠肝炎病毒、鼠痘病毒、小鼠肺炎病毒和呼肠孤病毒Ⅲ型)的阳性血清发生交叉反应,批内和批间重复性试验的变异系数均小于7%,与中国药品生物制品检定所的试剂盒符合率为98%。本研究建立的检测仙台病毒抗体的间接ELISA诊断方法,有很好的特异性和敏感性,为仙台病毒的抗体的检测提供了一种快速、简便的血清学诊断方法。
Using the recombinant and purified F（S） protein as antigen expressed in E. coli M15 and the anti-serum to F（S） protein as antibody, an indirect ELISA was successfully developed to detect antibody against Sendal virus. The recombinant F（S） protein antigen showed no cross-reaction with the positive sera of other 4 kinds of viruses containing MHV, Reo-3, ECT and PVM in mouse; coefficient of variability percent （C.V%） of intro-bateh duplieativity test and inter-batch duplieafivity test was less 7%. Result of comparison was tested between rf-ELISA and ELISA kit from National Institute For the Control of Pharmaceutical and Biological Products （NICPBP） showed that 98% concordance was obtained. Therefore, this rf-ELISA based on recombinant F（S） protein antigen has good sensitivity and specificity, can provide a simple and rapid assay for the antibody of Sendai virus.
出处
《上海交通大学学报（农业科学版）》
2008年第2期114-118,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科技发展基金项目(034909008)
