摘要
目的探讨癫痫发作后mGLuR1α的表达及其与神经元凋亡的关系,并明确mGLuR1α在癫痫活动中的作用。方法将大鼠随机分为致痫后6、12、24、48、72h组及对照组,应用免疫组织化学和RT-PCR方法检测戊四氮(PTZ)诱导大鼠癫痫发作后不同时间点脑神经细胞mGLuR1α的表达变化,采用原位末端标记法和流式细胞仪检测其神经元凋亡情况。结果(1)致痫后6hTUNEL染色阳性神经元开始表达,致痫后l2h最明显,24h开始下降,48h仍有表达。致痫后12h细胞凋亡率最高,之后随时间延长逐渐下降。(2)致痫后6hmGLuR1αmRNA转录水平即达最高,12h仍处于较高水平,之后逐渐降低,72h时仍高于正常水平,而受体的蛋白表达则稍晚,于致痫后l2h达到高峰,24h开始下降。(3)致痫大鼠mGLuR1α受体表达的最早和高峰时间与TUNEL反应时间基本吻合。结论mGLuR1α表达与致痫大鼠的神经细胞凋亡机制有关,癫痫发作可导致mGLuR1α一过性高表达,从而造成神经元损伤。
Objective To study the relationship between expression of mGLuR1α and neuron apoptosis in the rat brain with PTZ-induced seizures and identify the role of mGLuR1α on PTZ induced seizures. Methods The rats were randomly divided into the control group and 6, 12, 24, 48, 72 h group after PTZ-induced seizures. Immunohistochemical methods and RT-PCR technique were used to measure the expression of mGLuR1α in neurons at different point of time after PTZ-induced seizures, TUNEL technique and flow cytometry were used to measure the change trends of neuronal apoptosis. Results （1） TUNEL positive neurons began to express at 6 h following epilepsy, apoptotic neurons increased significantly at 12 h, began to decrease at 24 h, and were still in the state of downtrend at 72 h. The apoptotic ratio of the control group and PTZ group also showed the highest point at 12 h after PTZ-induced seizures, and fell-off with time lasting. （2） mGLuR1α mRNA transcription achieved the highest level at 6 h, and fell off following time lasting, it was still higher than control group at 72 h. But the expression of mGLuR1α protein was slightly late, it reached the peak at 12 h, and began to decrease at 24 h. （3） The earliest and peak time of mGLuR1α expression were basally coincidence with the reaction time of apoptotic neurons. Conclusions The expression of mGLuR1α was related to the mechanism of the neuronal apoptosis, PTZ-induced seizures resulted in the temporarily high expression of mGLuR1α, and so may lead to the neuronal damage.
出处
《中国神经免疫学和神经病学杂志》
CAS
2008年第3期167-170,178,I0002,共6页
Chinese Journal of Neuroimmunology and Neurology
