摘要
目的建立发育期戊四氮(PTZ)点燃癫大鼠模型,观察银杏叶提取物(EGb)对发育期点燃癫大鼠的干预作用,并探讨其干预机制,为拓展EGb的临床应用提供实验依据。方法 40只健康21日龄SD大鼠随机分成5组:正常对照组、PTZ+50 mL.L-1乙醇7 d组、PTZ+50 mL.L-1乙醇14 d组、PTZ+EGb 7 d组、PTZ+EGb 14 d组,每组8只。观察点燃及治疗过程中各组大鼠的发作情况,以Y型-电迷宫及Morris水迷宫评价各组大鼠的认知功能,并以病理方法评价海马神经细胞凋亡的情况。采用SPSS 16.0软件进行统计学分析。结果 EGb治疗后点燃癫大鼠癫发作的次数减少,Y型-电迷宫实验中达标次数明显减少,Morris水迷宫实验中逃避潜伏期明显缩短,穿越平台次数明显增多,原位末端标记(TUNEL)显示实验中海马CA3区神经细胞凋亡明显减少,但是EGb治疗14 d组与7 d组比较差异无统计学意义。结论 EGb治疗7 d和14 d均可改善发育期点燃癫大鼠的认知功能,但是并未显示出14 d疗程比7 d疗程有明显的疗效优势,此药理作用可能与减少或阻断海马神经细胞凋亡有关。
Objective To establish the pentylenetetrazol（PTZ） kindling models of developing rat model,to investigate the interventional effects of extracts of Ginkgo biloba leaves（EGb） on PTZ kindling models of developing rats and to discuss the underlying interventional mec-hanism so as to provide experimental basis for the development of clinical applications of EGb.Methods Forty healthy 21-day SD rats were randomly divided into 5 groups（n=8 for each group）：normal control group,PTZ＋50 mL·L^-1 ethanol 7 d group,PTZ＋50 mL·L^-1 ethanol 14 d group,PTZ＋EGb 7 d group,PTZ＋EGb 14 d group.The seizures were observed in each group at the time of kindling and throughout the treatment.The cognitive functions in each group was assessed with Y-maze test and Morris water maze.The expression of neural cell apoptosis in the hippocampus was evaluated pathologically.SPSS 16.0 software was used to analyze the data.Results At the end of EGb treatment,there was a decrease in the epileptiform seizure frequency as well as a marked reduction in the frequency of standards compliance.In the Morris water maze test,the escape latency was significantly shortened,the frequency of platform-crossing significantly increased,and the number of apoptotic neurons significantly decreased in CA3 area,while there was no statistical difference between EGb-treated 14 d group and 7 d group.Conclusions EGb therapy for either 7 days or 14 days can ameliorate the cognitive functions of the developing rats with kindled epilepsy,but it does not indicate that 14-day regimen has significantly curative advantage over 7-day regimen,which may be attributed to the reduction or blockage of the apoptosis of hippocampal neurons.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第17期1351-1354,共4页
Journal of Applied Clinical Pediatrics
基金
江苏省脑病生物信息重点实验室开放研究课题(Jsbl0806)
关键词
银杏叶提取物
点燃癫模型
认知功能
凋亡
大鼠
发育期
extracts of Ginkgo biloba leaves
kindling epilepsy model
cognitive function
apoptosis
developing rat
