摘要
应用逆转录聚合酶链反应（RT-PCR）技术，对C.jacchus猴实验性过敏性脑脊髓炎（EAE）脑组织中肿瘤坏死因子α（TNFα）mRNA的水平进行了研究。结果表明:EAE动物脑组织TNFαmRNA明显高于正常动物;用一种TNFα释放抑制剂环戊苯吡酮（rolipram）可阻断免疫动物EAE的发生和脑组织中TNFαmRNA水平降低。说明TNFα在EAE发生过程中具重要作用。采用抑制TNFα的手段，为多发性硬化症及相关疾病的治疗提供了新线索。
The objective of this work is to characterize the tumor necrosis factor-α(TNF-α) gene expression in the brain of experimental allergic encephalomyelitis (EAE).Methods To TNF-α gene expression in the brains from jacchus monkeys were characterized with 9 brain samples from EAE, 7 brain samples from Normal control, and 5 brain samples from rolipram treated or non-treated group, was employed to polymerase chain reaction (PCR) to approach for reverse transcribed RNA directly isolated from brain frozen sections.PCR products were quantified with an internal control. Results Results showed that TNF-α mRNA levels in the brain of EAE animals were higher than those of normal animals ; The release of TNF-α by Rolypram was suppressed, the TNF-α mRNA levels were reduced and clinical signs of EAE were inhibited. Conclusion These results suggest that TNF-α plays an important role in the course of EAE. The inhibition of TNF-α could be a potential means to approach for the treatment of demyelination disease.
出处
《中国神经免疫学和神经病学杂志》
CAS
1997年第2期63-67,共5页
Chinese Journal of Neuroimmunology and Neurology
基金
香港
美国加州大学旧金山医学院郑氏医学奖学金资助
关键词
脑脊髓炎
聚合酶链反应
肿瘤坏死因子Α
tumor necrosis factor-α
experimental allergic encephalomyelitis
reverse transcriptase-PCR
