摘要
目的研究三味檀香散对异丙肾上腺素(Isoprotere-nol,Iso)致大鼠实验性心肌缺血的保护作用,并探讨其可能的作用机制。方法采用腹腔注射(ip)Iso诱导大鼠造成心肌缺血的动物模型(Iso5mg·kg-1,每日1次,连续3d),末次给Iso后24h,大鼠颈动脉取血,测定血清乳酸脱氢酶(LDH)和肌酸激酶(CK)。实验结束后,留取心脏并测定心肌组织一氧化氮合酶(NOS)、诱导型一氧化氮合酶(iNOS)、一氧化氮(NO)和心肌梗死范围大小,并电镜观察心肌组织超微结构变化。结果预先给予三味檀香散组血清LDH和CK活性明显低于模型对照组;心肌组织中NOS、iNOS、NO和心肌梗死范围均明显低于模型对照组;心肌组织电镜观察表明:三味檀香散组心肌组织超微结构损伤明显轻于模型对照组,心肌纤维排列整齐,肌膜平整光滑,线粒体嵴密集、排列整齐,肿胀及空泡明显减轻。结论藏药三味檀香散对Iso所致大鼠心肌缺血损伤具有一定的保护作用,而抗心肌缺血的机制可能与其保护心肌线粒体结构,改善缺血心肌的能量代谢和降低心肌中NOS和iNOS的活性,减少NO过量释放有关。
Ahn To explore protective effect of Sanweitanxiang powder on isoproterenol-induced myocardial ischemia in rats, and its possible mechanism. Methods A myocardial ischemia model in rats was established by intraperitoneal injection of isoproterenol （ Iso 5 mg · kg ^-1 once a day , for 3 days）.After ip 24 h, The levels of LDH, CK in serum and NOS, iNOS and NO in the myocardium tissue were measured. The myocardial area suffered from ischemia injury were determined. The changes of myocardial uhrastructure were observed with electron microscope. Results Compared with model group, the levels of LDH, CK and infarction size of myocardium were decreased. The concentrations of NOS, iNOS and NO in myocardium were less than those in the model group. The results of myocardial ultrastructure indicated that the partial mitochondria crista and myofibrils sprase, z band unaltered in Sanweitanxiang powder groups. Conclusion Sanweitanxiang powder has a significant protective effect on isoproterenol-induced myocardial injury in rats. By improving the energy metabolism of myocardium,the structure and function of mitochondria and re- ducing the NO release.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第4期485-488,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30540020)
关键词
三味檀香散
异丙肾上腺素
心肌缺血
线粒体
一氧化氮
Sanweitanxiang powder
isoproterenol
myocardial injury
mitochondria
nitric oxide
