摘要
目的探讨Nogo-A蛋白在新生大鼠缺氧缺血性脑损伤脑组织中的表达规律及意义。方法将7日龄新生Wistar大鼠96只随机分为对照组(n=48)和实验组(n=48),实验组制成缺氧缺血性脑损伤(HIBD)模型,根据处死动物的时间点不同再随机分为HIBD后1,4,7,10,14,21d共6个亚组,每组8只;对照组也在相应的时间点取材,每个时间点8只;采用免疫组化法检测Nogo-A蛋白在不同时间点的表达。结果缺氧缺血后新生大鼠出现不同程度的行为异常。Nogo-A蛋白平均灰度值在实验组术后第1,4,7,10,14,21天较对照组均降低,同一时间点实验组与对照组差异均有统计学意义(P<0.05)。且随着日龄的增加,Nogo-A蛋白平均灰度值呈逐渐降低的趋势。结论Nogo-A蛋白广泛表达于大脑皮层,缺氧缺血性脑损伤后其表达增强,且长时间持续于高水平,可能是造成新生大鼠缺氧缺血性脑损伤后神经再生障碍的重要原因之一。
Objective To probe the principle and significance of expression of nerve growth inhibitor NOgo-A in brain of neonatal rats with hypoxic-isehemic brain damage（HIBD）. Metkods Ninety-six 7-day-old Wistar rats were randomly divided into normal control group（ n = 48）and experimental group（ n = 48）. Each group was divided into six subgroups（ n = 8 in each subgroup）based on different time points after HIBD（at 1,4,7,10,14,21 d after HIBD）. The pathological changes in brain were observed under optical microscope, and the expression of nerve growth inhibitor Nogo-A in cerebral cortex was examined with immunohistochemical staining and image quantitative analysis at each time point. Results Neonatal rats had different degrees of behavior disorder after HIBD. The value of average gray scale of Nogo-A-positive cells in cerebral cortex in experimental group at 1,4,7,10,14,21 d after HIBD was significantly lower than in control group（ P 〈 0.01）. Conclusion The expression of Nogo-A increases in cerebral cortex of neonatal rats with HIBD, and keeps a higher level for a long time, which may be an important factor for the disorder of neural regeneration of HIBD rats.
出处
《山西医科大学学报》
CAS
2008年第4期324-326,385,共4页
Journal of Shanxi Medical University
基金
太原市科技局科技兴市专项基金资助项目(0705026)
